Böhn I, Niessen K H, Reineke F, Teufel M
Universitäts-Kinderklinik Mannheim.
Klin Padiatr. 1994 Mar-Apr;206(2):95-9. doi: 10.1055/s-2008-1046590.
The anions analysis was a methodical problem up to now. This was the reason for low interest. Biological fluids like saliva and urine which could easily receive without any stress for the children, are little investigated for its capacity on nitrite, nitrate, bromide and sulfate. In this performance there will presented an ion-chromatographic method to determine inorganic anions in the following body-fluids: serum saliva, liquor and urine. The anions chloride, nitrite, bromide, nitrate, phosphate and sulfate was determined quantitatively. The method was proved in a pilot-study on children's body-fluids serum, liquor and saliva. The objects was to get a landmark in expectation from anion concentrations. Bromide was detected as a constant part in all body fluids. The origin and importance is not clear till now. Also was found nitrate in all investigated body fluids. There seems to be a connection between diarrhea and an increase in serum levels from nitrate. We found considerable amounts of nitrate in saliva by babies and infants. The method is distinguished by little fluctuation in measurement and high specificity. Short time in analysis and simple handling will do the method for a qualified one in pediatrics.
到目前为止，阴离子分析一直是个系统性问题。这就是人们对此兴趣不高的原因。像唾液和尿液这样的生物体液，孩子们可以轻松获取，且不会有任何压力，但对其亚硝酸盐、硝酸盐、溴化物和硫酸盐含量的研究却很少。在本研究中，将介绍一种离子色谱法，用于测定以下体液中的无机阴离子：血清、唾液、脑脊液和尿液。对氯离子、亚硝酸盐、溴化物、硝酸盐、磷酸盐和硫酸盐等阴离子进行了定量测定。该方法在一项针对儿童体液（血清、脑脊液和唾液）的初步研究中得到了验证。目的是从阴离子浓度中找到一个标志性数值。在所有体液中都检测到溴化物是一个常量成分。其来源和重要性至今尚不清楚。在所有被研究的体液中也都发现了硝酸盐。腹泻与血清中硝酸盐水平升高之间似乎存在某种联系。我们在婴儿的唾液中发现了大量硝酸盐。该方法的特点是测量波动小、特异性高。分析时间短且操作简单，使其成为儿科领域一种合格的方法。
