Lambert P R
Department of Otolaryngology-Head and Neck Surgery, University of Virginia Health Science Center, Charlottesville 22908.
Laryngoscope. 1994 Jun;104(6 Pt 1):701-18. doi: 10.1288/00005537-199406000-00010.
Recent observations have shown that mammals possess a limited capacity for regeneration of inner ear sensory epithelia. It is clear, however, that a mitogenic growth factor will be necessary to up-regulate this capacity before clinical application becomes feasible. This study used in vitro cultures of adult mouse vestibular organs for assessing the mitogenic effect of transforming growth factor alpha (TGF-alpha). Sixty-one utricles and cristae were maintained in culture for 7 to 8 days. Neomycin was used to damage the hair cells. Autoradiography permitted identification of any cell which had undergone mitosis during the culture period. The proliferative response was compared in organs exposed to TGF-alpha and those maintained in the basic culture medium only. The results demonstrated that TGF-alpha significantly increased cell proliferation in the sensory epithelia and also in the marginal zones surrounding them. This finding provides a scientific basis for the concept that inner ear hair cell damage in humans may someday be reversible pharmacologically.
最近的观察结果表明，哺乳动物内耳感觉上皮的再生能力有限。然而，很明显，在临床应用可行之前，有丝分裂生长因子对于上调这种能力将是必要的。本研究使用成年小鼠前庭器官的体外培养物来评估转化生长因子α（TGF-α）的促有丝分裂作用。61个椭圆囊和嵴在培养中维持7至8天。使用新霉素损伤毛细胞。放射自显影术可识别培养期间发生有丝分裂的任何细胞。比较了暴露于TGF-α的器官和仅维持在基础培养基中的器官的增殖反应。结果表明，TGF-α显著增加了感觉上皮及其周围边缘区域的细胞增殖。这一发现为人类内耳毛细胞损伤有朝一日可能通过药理学方法逆转这一概念提供了科学依据。
