Yamazaki K, Sato K, Hirata S, Ikeda K, Kubo Y, Matsui H, Ogawa K
First Department of Surgery, Asahikawa Medical College, Japan.
Nihon Geka Gakkai Zasshi. 1994 Apr;95(4):242-7.
We studied DNA ploidy, point mutation of Ki-ras oncogene codon 12, and p21 expression using paraffin embedded materials from 42 cases of colorectal cancer. DNA ploidy was measured by the method of Hedley et al. flow cytometrically. Point mutation of Ki-ras oncogene was examined by the method of Bos et al. using a dot-blot screening procedure, and p21 expression was examined immunohistochemically. Incidence of aneuploidy, Ki-ras point mutation, and p21 expression was 71.4%, 26.2%, 40.5%, respectively. There was a very weak correlation between p21 expression and pathologic findings, but there was no correlation between pathologic findings and DNA ploidy, as well as Ki-ras point mutation. Patients who showed aneuploidy tended to have more point mutation of Ki-ras oncogene. There was no correlation between p21 expression and Ki-ras point mutation, as well as DNA ploidy. Although there was no correlation between Ki-ras point mutation and survival, a significant correlation between survival and DNA ploidy, as well as p21 expression was recognized. Patients who had tumors with diploidy or p21 expression tended to have better prognosis.
我们使用42例结直肠癌石蜡包埋材料研究了DNA倍体、Ki-ras癌基因密码子12的点突变以及p21表达。DNA倍体采用Hedley等人的方法通过流式细胞术进行检测。Ki-ras癌基因的点突变采用Bos等人的方法通过斑点杂交筛选程序进行检测，p21表达采用免疫组织化学方法进行检测。非整倍体、Ki-ras点突变和p21表达的发生率分别为71.4%、26.2%、40.5%。p21表达与病理结果之间存在非常微弱的相关性，但病理结果与DNA倍体以及Ki-ras点突变之间无相关性。显示非整倍体的患者往往有更多的Ki-ras癌基因点突变。p21表达与Ki-ras点突变以及DNA倍体之间无相关性。虽然Ki-ras点突变与生存率之间无相关性，但生存率与DNA倍体以及p21表达之间存在显著相关性。肿瘤为二倍体或有p21表达的患者往往预后较好。
