Lindley C, Sawyer W, Haddon T, Meade J, Nolen J, Johansen L, Roberts H
University of North Carolina, Chapel Hill.
Pharmacotherapy. 1994 Mar-Apr;14(2):224-8.
To compare prothrombin time (PT), activated partial thromboplastin time (aPTT), and factor VII values in concurrent blood samples obtained by direct venipuncture and from a peripheral venous catheter.
Concurrent samples obtained from catheters and by direct venipuncture were studied. In a separate crossover bioequivalence assessment of DNA-derived factor VIIa (rFVIIa) from two different batches, sample results of each technique were compared.
University hospital clinical research unit.
Six patients with hemophilia A under nonbleeding conditions.
The patients received a single dose of rFVIIa 70 micrograms/kg administered by intravenous push over 2 minutes. Concurrent blood samples were collected at 2, 3, 4, 6, 8, 10, and 12 hours after rFVIIa administration. Catheter blood samples were drawn from a three-way stopcock attached to an 18-gauge peripheral venous catheter in the patient's forearm and connected to an intravenous solution of 5% dextrose with half normal saline maintained at a rate of 30 ml/hour. Venipuncture samples were drawn from the opposite arm.
The PT and aPTT values were determined by using a BBL Fibrometer (PT) and a Coagamate X-2 with automated aPTT reagent. Blood samples were analyzed for factor VII concentration using the Novo Clot assay. The mean venipuncture-obtained PT (8.9 +/- 1.0 sec) and aPTT (48.7 +/- 13.6 sec) values were numerically equivalent to mean catheter-derived PT (9.0 +/- 1.0 sec) and aPTT (48.3 +/- 12.5 sec) results, as were mean venipuncture and catheter-obtained FVII:C values.
The PT and aPTT values determined after venipuncture and through the peripheral catheter were not statistically different (p > 0.05) when compared by paired or unpaired analysis. Similarly, values of FVII:C measured after venipuncture were statistically equivalent to those after sampling through the peripheral catheter. All six patients preferred the catheter method of blood collection over venipuncture.
比较通过直接静脉穿刺和外周静脉导管采集的同期血样中的凝血酶原时间（PT）、活化部分凝血活酶时间（aPTT）及因子VII值。
对通过导管和直接静脉穿刺采集的同期样本进行研究。在对来自两个不同批次的DNA衍生因子VIIa（重组FVIIa）进行单独的交叉生物等效性评估时，比较了每种技术的样本结果。
大学医院临床研究单位。
6名处于非出血状态的甲型血友病患者。
患者接受单次剂量70微克/千克的重组FVIIa，通过静脉推注在2分钟内给药。在重组FVIIa给药后2、3、4、6、8、10和12小时采集同期血样。导管血样从前臂18号外周静脉导管连接的三通旋塞抽取，连接至以30毫升/小时速度维持的5%葡萄糖与半量生理盐水的静脉溶液。静脉穿刺血样从对侧手臂抽取。
PT和aPTT值使用BBL纤维蛋白测定仪（PT）和配备自动aPTT试剂的Coagamate X-2进行测定。使用诺和凝血检测法分析血样中的因子VII浓度。静脉穿刺获得的平均PT（8.9±1.0秒）和aPTT（48.7±13.6秒）值在数值上与导管获得的平均PT（9.0±1.0秒）和aPTT（48.3±12.5秒）结果相当，静脉穿刺和导管获得的FVII:C平均值也是如此。
通过配对或非配对分析比较时，静脉穿刺和通过外周导管测定的PT和aPTT值无统计学差异（p>0.05）。同样，静脉穿刺后测得的FVII:C值与通过外周导管采样后测得的值在统计学上相当。所有6名患者都更倾向于通过导管采血而非静脉穿刺。
