Ozesmi C, Aydogan S, Ascioglu M
Department of Physiology, Faculty of Medicine, Erciyes University, Kayseri, Turkey.
Vet Hum Toxicol. 1994 Apr;36(2):117-9.
The presence of grayanotoxins (GTX-I, GTX-II and GTX-III) in honey samples from the Mediterranean Sea and Black Sea regions of Turkey and the effect of honey containing GTX-I on the threshold stimulus voltage of frog gastrocnemius muscle were studied. We used 2 groups of gastrocnemius muscles isolated from curarized or noncurarized frogs. The threshold voltage was measured by external perfusion with a polygraphic system. Isolated muscle preparations were perfused separately with Clark-frog-Ringer's solution containing honey solutions from the Mediterranean Sea region (normal) or with honey solutions from the Black Sea region (GTX-I-containing, poisonous). There was no significant difference between the normal and poisonous honey groups data with the curarized preparations. But in the noncurarized preparations, the honey containing GTX-I significantly decreased the threshold voltage in comparison with normal honey. These findings suggest the effect of GTX-I on frog neuromuscular junctions is due to an increase in membrane permeability to sodium ions.
对土耳其地中海和黑海地区蜂蜜样本中灰毒素（GTX-I、GTX-II和GTX-III）的存在情况以及含GTX-I的蜂蜜对青蛙腓肠肌阈刺激电压的影响进行了研究。我们使用了两组从箭毒化或未箭毒化青蛙分离出的腓肠肌。通过多导记录系统进行外部灌注来测量阈电压。分离的肌肉标本分别用含有来自地中海地区蜂蜜溶液（正常）的克拉克青蛙林格氏液或来自黑海地区蜂蜜溶液（含GTX-I，有毒）进行灌注。箭毒化标本的正常蜂蜜组和有毒蜂蜜组数据之间没有显著差异。但在未箭毒化标本中，与正常蜂蜜相比，含GTX-I的蜂蜜显著降低了阈电压。这些发现表明GTX-I对青蛙神经肌肉接头的作用是由于膜对钠离子的通透性增加。
