Kubota T
Department of Ophthalmology, Shinshu University School of Medicine, Nagano-ken, Japan.
Nippon Ganka Gakkai Zasshi. 1994 May;98(5):429-34.
We determined the protein profiles of the trabecular meshwork (TM) obtained from 14 normal human eyes from individuals of various ages (53 to 76 years) using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a highly sensitive silver staining technique, which yields 50-100 times greater sensitivity than is achieved with Coomassie Blue. Over 30 protein bands varying in molecular weight from 16,000 to 250,000 were consistently visible, regardless of donor age. Protein profiles of one-eighth human TM obtained from each eye showed a same pattern and those of one-eight organ-cultured human TM during 4 weeks also indicated a same pattern. Protein profiles of the TM from normal human eyes of various ages shared a number of similarities. Protein profiles of the TM, peripheral cornea, limbal sclera, iris, and ciliary body from a control donor were similar, although several polypeptide bands were unique to each structure.
我们使用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和一种高灵敏度银染技术，测定了取自14只不同年龄（53至76岁）正常人眼小梁网（TM）的蛋白质谱，该银染技术的灵敏度比考马斯亮蓝高50至100倍。无论供体年龄如何，始终可见超过30条分子量在16,000至250,000之间变化的蛋白条带。从每只眼睛获取的八分之一人小梁网的蛋白质谱显示出相同的模式，并且在4周的器官培养人小梁网的八分之一的蛋白质谱也显示出相同的模式。不同年龄正常人眼小梁网的蛋白质谱有许多相似之处。来自对照供体的小梁网、周边角膜、角膜缘巩膜、虹膜和睫状体的蛋白质谱相似，尽管每个结构都有几条独特的多肽条带。
