Toft E, Söderström M, Ahlberg M B, DePierre J W
Department of Biochemistry, Wallenberg Laboratory, Stockholm University, Sweden.
Biochem Biophys Res Commun. 1994 May 30;201(1):149-54. doi: 10.1006/bbrc.1994.1681.
A novel protein that binds to a glutathione-Sepharose affinity column has been detected in mature, but not immature, rat ovary. This protein could be resolved from all identifiable components when the affinity-purified material, containing primarily glutathione transferases, was analyzed on reversed phase-HPLC. The unidentified protein migrated with an apparent molecular weight of 34 kDa on SDS-PAGE. After purification by affinity chromatography and subsequent preparative electrophoresis, the protein was subjected to N-terminal amino acid sequence analysis. The sequence obtained demonstrated a high degree of homology with an internal amino acid sequence in human carbonyl reductase (EC 1.1.1.184).
