Sato H, Yamaguchi M, Bannai S
Department of Biological Chemistry, University of Tsukuba, Ibaraki, Japan.
Biochem Biophys Res Commun. 1994 May 30;201(1):38-44. doi: 10.1006/bbrc.1994.1666.
Ferritin synthesis is known to be regulated translationally by specific mRNA-protein interactions between an iron-responsive element (IRE) localized in the 5' untranslated region of ferritin mRNA and IRE-binding protein (IRE-BP). Binding of IRE-BP to IRE depresses its translation. In the present study, we demonstrated that ferritin synthesis in macrophages is strongly induced under hypoxic conditions by diethylmaleate, a sulfhydryl-reactive agent. The induction by diethylmaleate decreased as the oxygen tension rose. O2- is involved in this oxygen effect, because the induction was prevented when O2(-)-generating agents were present. Actinomycin D did not inhibit the ferritin synthesis induced by diethylmaleate under hypoxi. These results suggest that O2- is involved in post-transcriptional regulation of ferritin synthesis.
已知铁蛋白的合成通过位于铁蛋白mRNA 5'非翻译区的铁反应元件（IRE）与IRE结合蛋白（IRE-BP）之间的特定mRNA-蛋白质相互作用进行翻译调控。IRE-BP与IRE的结合会抑制其翻译。在本研究中，我们证明，巯基反应剂马来酸二乙酯在低氧条件下可强烈诱导巨噬细胞中铁蛋白的合成。随着氧张力升高，马来酸二乙酯的诱导作用降低。超氧阴离子（O2-）参与了这种氧效应，因为当存在产生O2-的试剂时，诱导作用被阻止。放线菌素D在低氧条件下不抑制马来酸二乙酯诱导的铁蛋白合成。这些结果表明，O2-参与了铁蛋白合成的转录后调控。
