Stark G B, Kaiser H W
Department of Plastic and Reconstructive Surgery, Cologne Medical Centre Merheim, Germany.
Burns. 1994;20 Suppl 1:S34-8. doi: 10.1016/0305-4179(94)90087-6.
Autologous keratinocytes cultured in vitro from skin biopsies of patients with deep partial and full skin thickness burns were grafted onto nine necrectomized wound surfaces between 17 and 25 days after injury. The cells were applied as nonconfluent single cells suspended in fibrin glue. In four wounds, this cell-fibrin suspension was used to attach an additional glycerolized allogeneic split thickness skin graft (STSG). Re-epithelialization was very rapid as demonstrated clinically and histologically. Keratinocyte grafted areas without cadaver skin overgraft showed less mechanical stability than when the keratinocyte-fibrin glue suspension was combined with allogeneic STSG. There is clinical and histological evidence that the allodermis may be partially integrated into the new skin.
从深度部分和全层皮肤烧伤患者的皮肤活检组织中体外培养的自体角质形成细胞，在受伤后17至25天被移植到9个清创后的创面。细胞以悬浮于纤维蛋白胶中的未融合单细胞形式应用。在4个创面上，这种细胞-纤维蛋白悬液被用于附着额外的甘油化异体中厚皮片（STSG）。临床和组织学结果均显示，再上皮化过程非常迅速。未覆盖尸体皮肤的角质形成细胞移植区域，其机械稳定性低于角质形成细胞-纤维蛋白胶悬液与异体STSG联合使用时的情况。有临床和组织学证据表明，异体真皮可能会部分整合到新皮肤中。
