McKay I, Woodward B, Wood K, Navsaria H A, Hoekstra H, Green C
Blond McIndoe Center for Medical Research, East Grinstead, Sussex, UK.
Burns. 1994;20 Suppl 1:S19-22. doi: 10.1016/0305-4179(94)90083-3.
The aim of this project was to reconstruct human skin from glycerol-preserved dermis and layers of cultured keratinocytes for use in the treatment of deep burns and ulcers. Glycerol-preserved cadaver skin from the Euro Skin Bank was treated with Dispase II or PBS, under various conditions, to find the best method of retaining the physical structure of the dermis while removing the epidermis and remnants of dead dermal cells which might provoke an allogeneic reaction in a graft recipient. Monoclonal antibodies LH39 and LH7.2, with specificity for basement membrane determinants, showed that treatment with Dispase II resulted in separation of the epidermis from the dermis with concomitant loss of all cellular elements from the dermal layer (as judged by H and E staining). However, when sheets of cultured keratinocytes were applied to the treated dermis and cultured for several days, the keratinocytes attached and regenerated a new basement membrane.
该项目的目的是利用甘油保存的真皮和培养的角质形成细胞层重建人皮，用于治疗深度烧伤和溃疡。对来自欧洲皮肤库的甘油保存尸体皮肤在各种条件下用Dispase II或PBS进行处理，以找到在去除表皮和可能在移植受者中引发同种异体反应的死亡真皮细胞残余物的同时保留真皮物理结构的最佳方法。对基底膜决定簇具有特异性的单克隆抗体LH39和LH7.2表明，用Dispase II处理导致表皮与真皮分离，同时真皮层所有细胞成分丧失（通过苏木精和伊红染色判断）。然而，当将培养的角质形成细胞片应用于处理过的真皮并培养数天时，角质形成细胞附着并再生了新的基底膜。
