Sjöberg A M, Alanko T A
VTT Biotechnology and Food Research, Espoo, Finland.
J AOAC Int. 1994 Mar-Apr;77(2):425-30.
A collaborative study was conducted of a spectrophotometric method for determination of nitrate after cadmium reduction to nitrite in baby foods containing meat. Thirty-one municipal and 2 industrial food laboratories participated in the study. The study design involved 2 baby food matrices. Samples of both matrices were prepared at 3 concentration levels between 52 and 309 mg NaNO3/kg as blind duplicates. A blank without added nitrate was also included. The outlier percentage of the results was very low (4.3%). It was typical for the method that recoveries were slightly > 100%. Recoveries for baby foods varied between 113.3 and 116.9%, and were acceptable for control purposes. The relative standard deviations for repeatability were 5.0-18.1%. The relative standard deviations for reproducibility were 8.3-21.6%. Three collaborators also evaluated liquid chromatographic technique for nitrate determinations. These preliminary results are presented but are not analyzed statistically. The spectrophotometric method was adopted first action by AOAC INTERNATIONAL.
开展了一项合作研究，该研究针对一种分光光度法，用于测定肉类婴儿食品中镉还原为亚硝酸盐后的硝酸盐含量。31家市级食品实验室和2家工业食品实验室参与了此项研究。研究设计涉及2种婴儿食品基质。两种基质的样品均在52至309 mg NaNO₃/kg之间的3个浓度水平下制备，作为盲样重复样品。还包括未添加硝酸盐的空白样品。结果的异常值百分比非常低（4.3%）。该方法的典型情况是回收率略大于100%。婴儿食品的回收率在113.3%至116.9%之间，用于控制目的是可以接受的。重复性的相对标准偏差为5.0 - 18.1%。再现性的相对标准偏差为8.3 - 21.6%。三位合作者还评估了用于硝酸盐测定的液相色谱技术。给出了这些初步结果，但未进行统计分析。该分光光度法被美国官方分析化学师协会（AOAC INTERNATIONAL）首次采用。
