基因毒性致癌物六价铬在体内可改变金属硫蛋白基因的金属诱导型表达，但不改变其基础表达。
The genotoxic carcinogen chromium(VI) alters the metal-inducible expression but not the basal expression of the metallothionein gene in vivo.
作者信息
Alcedo J A, Misra M, Hamilton J W, Wetterhahn K E
机构信息
Department of Biochemistry, Dartmouth Medical School, Hanover, NH.
出版信息
Carcinogenesis. 1994 May;15(5):1089-92. doi: 10.1093/carcin/15.5.1089.
The ability of the carcinogen chromium(VI) to affect the basal and zinc-inducible expression of liver metallothionein was examined in 14- and 18-day chicken embryos in vivo. Metallothionein expression varied with the stage of embryo development, with basal steady-state mRNA levels being approximately three times lower in livers of 18-day versus 14-day chicken embryos. Chromium(VI) treatment had no effect on the basal steady-state levels of metallothionein mRNA and protein in either 14- or 18-day chicken embryo liver. Treatment of 14-day embryos with zinc(II) resulted in a 3- to 5-fold increase in steady-state levels of metallothionein mRNA in liver. Pre-treatment of 14-day embryos with chromium(VI) inhibited the zinc(II)-induced increase in steady-state levels of metallothionein mRNA and protein in liver by 30-50%. In contrast, chromium(VI) and/or zinc(II) treatments had no effect on steady-state levels of beta-actin mRNA.
在14日龄和18日龄鸡胚体内，研究了致癌物六价铬影响肝脏金属硫蛋白基础表达及锌诱导表达的能力。金属硫蛋白的表达随胚胎发育阶段而变化，18日龄鸡胚肝脏中基础稳态mRNA水平比14日龄鸡胚肝脏中的低约三倍。六价铬处理对14日龄或18日龄鸡胚肝脏中金属硫蛋白mRNA和蛋白质的基础稳态水平均无影响。用锌（II）处理14日龄胚胎，导致肝脏中金属硫蛋白mRNA稳态水平增加3至5倍。用六价铬预处理14日龄胚胎，可使锌（II）诱导的肝脏中金属硫蛋白mRNA和蛋白质稳态水平增加受到30%至50%的抑制。相比之下，六价铬和/或锌（II）处理对β-肌动蛋白mRNA的稳态水平没有影响。
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