Nukada H, Simpson L O, Robertson A M
Department of Medicine, University of Otago Medical School, Dunedin, New Zealand.
Diabetes Res. 1993;22(1):21-32.
Abnormalities of blood rheology may play an important role in the underlying mechanism of the development of diabetic complications. Although streptozotocin (STZ)-induced diabetic rats have been used extensively in the investigation of diabetic complications, blood rheological alterations in this model have not previously been assessed. To assess one aspect of blood rheology, we repeated whole blood viscosity (WBV) measurements at 4, 8, 12 and 16 weeks after the STZ injection in rats. Nine of fifteen diabetic rats showed increased WBV after the induction of diabetes. Although these rats had a higher haematocrit, the increase in WBV was maintained after correction to a standard haematocrit. Percentage changes of WBV were significantly greater in these diabetic rats than in control rats at low shear rates (0.1s-1 and 1.0s-1), but not at high shear rates. In contrast, WBV was lowered at all shear rates after the injection of STZ in the remaining six diabetic rats which had lower haematocrit, severe weight loss and high mortality; no rats survived for 16 weeks post-injection. Animals in this group may be implicated in the high mortality among STZ-diabetic rats. Although there was a clear distinction of rheological and clinical pictures between these two groups of STZ-diabetic rats, blood glucose and HbA1 concentrations did not differ significantly. This study demonstrates a dichotomy in blood viscosity response to STZ-induced diabetes.
血液流变学异常可能在糖尿病并发症发生发展的潜在机制中起重要作用。尽管链脲佐菌素（STZ）诱导的糖尿病大鼠已广泛用于糖尿病并发症的研究，但该模型中的血液流变学改变此前尚未得到评估。为评估血液流变学的一个方面，我们在大鼠注射STZ后4、8、12和16周重复测量全血粘度（WBV）。15只糖尿病大鼠中有9只在糖尿病诱导后WBV升高。尽管这些大鼠的血细胞比容较高，但在校正至标准血细胞比容后，WBV的升高仍持续存在。在低剪切率（0.1s-1和1.0s-1）下，这些糖尿病大鼠的WBV百分比变化显著大于对照大鼠，但在高剪切率下则不然。相比之下，其余6只血细胞比容较低、体重严重减轻且死亡率高的糖尿病大鼠在注射STZ后，所有剪切率下的WBV均降低；注射后无大鼠存活至16周。该组动物可能与STZ诱导的糖尿病大鼠的高死亡率有关。尽管这两组STZ诱导的糖尿病大鼠在流变学和临床表现上有明显区别，但血糖和糖化血红蛋白（HbA1）浓度并无显著差异。本研究表明，对STZ诱导的糖尿病，血液粘度反应存在二分法。
