McHale M, Coldwell M C, Herrity N, Boyfield I, Winn F M, Ball S, Cook T, Robinson J H, Gloger I S
Department of Biotechnology, SmithKline Beecham Pharmaceuticals, Harlow, Essex, UK.
FEBS Lett. 1994 May 30;345(2-3):147-50. doi: 10.1016/0014-5793(94)00423-4.
A synthetic version of the human D4 (hD4) dopamine receptor was prepared. The G/C content of the natural gene was reduced by 14% without altering the amino acid composition of the corresponding protein sequence. HEK293 cells were transfected with the synthetic hD4 gene and stable clones resistant to G418 selected. The hD4 receptor expressed from the synthetic gene had identical pharmacological characteristics to the native hD4 receptor [(1991) Nature 350, 610-619; (1992) Nature 358, 149-152]. Functional studies with cells expressing the synthetic hD4 gene indicated negative coupling of this receptor to adenylate cyclase.
制备了人D4（hD4）多巴胺受体的合成版本。天然基因的G/C含量降低了14%，而相应蛋白质序列的氨基酸组成未改变。用合成的hD4基因转染HEK293细胞，并筛选出对G418有抗性的稳定克隆。从合成基因表达的hD4受体具有与天然hD4受体相同的药理学特性[（1991年）《自然》350卷，610 - 619页；（1992年）《自然》358卷，149 - 152页]。对表达合成hD4基因的细胞进行的功能研究表明，该受体与腺苷酸环化酶呈负偶联。
