Santos O F, Barros E J, Yang X M, Matsumoto K, Nakamura T, Park M, Nigam S K
Harvard Medical School, Boston, Massachusetts 02115.
Dev Biol. 1994 Jun;163(2):525-9. doi: 10.1006/dbio.1994.1169.
Using PCR, hepatocyte growth factor (HGF) and c-met (HGF receptor) expression were analyzed in the developing mouse kidney. Both HGF and c-met were expressed from Gestational Day 11.5 onward, the time at which branching morphogenesis of ureteric bud occurs. Coculturing the embryonic kidney with MDCK cells seeded in Type I collagen induced branching morphogenesis of the MDCK cells. When a monospecific antiserum against HGF was included in the culture medium, the branching morphogenesis induced by the embryonic kidney was markedly inhibited (73%). This anti-HGF serum also inhibited metanephric growth when incubated with 11.5- to 12.5-day embryonic kidneys in an organ culture assay. No inhibition was observed by nonimmune serum. Perturbation of ureteric duct development as well as extraductal metanephric development was observed in embryonic kidneys incubated with the anti-HGF serum. Together, our data indicates an important role for HGF in kidney development.
利用聚合酶链反应（PCR），对发育中的小鼠肾脏中肝细胞生长因子（HGF）和c-met（HGF受体）的表达进行了分析。HGF和c-met从妊娠第11.5天起开始表达，此时输尿管芽开始发生分支形态发生。将胚胎肾脏与接种在I型胶原中的MDCK细胞共培养，可诱导MDCK细胞发生分支形态发生。当培养基中加入抗HGF单特异性抗血清时，胚胎肾脏诱导的分支形态发生受到明显抑制（73%）。在器官培养试验中，这种抗HGF血清与11.5至12.5天的胚胎肾脏一起孵育时，也会抑制后肾生长。非免疫血清未观察到抑制作用。在用抗HGF血清孵育的胚胎肾脏中，观察到输尿管发育以及肾外后肾发育受到干扰。总之，我们的数据表明HGF在肾脏发育中起重要作用。
