通过随机扩增多态性DNA分析确定入侵害虫阿根廷茎象甲（Listronotus bonariensis (Kuschel)）的地理起源。
Geographical origin of an introduced insect pest, Listronotus bonariensis (Kuschel), determined by RAPD analysis.
作者信息
Williams C L, Goldson S L, Baird D B, Bullock D W
机构信息
Centre for Molecular Biology, Lincoln University, Canterbury, New Zealand.
出版信息
Heredity (Edinb). 1994 Apr;72 ( Pt 4):412-9. doi: 10.1038/hdy.1994.57.
The Argentine stem weevil, Listronotus bonariensis (Kuschel) (Coleoptera: Curculionidae), is an important introduced pasture pest in New Zealand. In this study geographical populations of this species were analysed using polymerase chain reaction-based randomly amplified polymorphic DNA (RAPD), in an attempt to determine the geographical origin of the pest. Morphologically indistinguishable individuals were collected from nine South American, five New Zealand and one Australian populations. Ten primers were screened for usefulness, two of which revealed significant, scorable polymorphisms between these populations. The results indicated that the sampled New Zealand L. bonariensis populations originated from the east coast of South America.
阿根廷茎象甲，Listronotus bonariensis（库舍尔）（鞘翅目：象甲科），是新西兰一种重要的外来牧场害虫。在本研究中，利用基于聚合酶链反应的随机扩增多态性DNA（RAPD）分析了该物种的地理种群，试图确定该害虫的地理起源。从9个南美种群、5个新西兰种群和1个澳大利亚种群中收集了形态上无法区分的个体。筛选了10种引物的有效性，其中2种引物在这些种群之间显示出显著的、可评分的多态性。结果表明，采样的新西兰博纳里茎象甲种群起源于南美洲东海岸。
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