血红蛋白对微粒体苯胺和乙酰苯胺羟化作用的增强

Enhancement of microsomal aniline and acetanilide hydroxylation by haemoglobin.

Jonen H G, Kahl R, Kahl G F

Xenobiotica. 1976 May;6(5):307-20. doi: 10.3109/00498257609151642.

Abstract

Haemogloblin and myoglobin enhance rat liver microsomal p-hydroxylation of aniline and acetanilide. Microsomal N-demethylation of ethylmorphine and aminopyrine is not increased by haemoproteins. 2. The enhancement of microsomal p-hydroxylation is maximal at high substrate concentration and high haeme compound concentration. 3. Detergent-purified NADPH-cytochrome c reductase, free flavins and manganese ions considerably increase the haemoglobin-mediated, tissue-free hydroxylation of aniline. Microsomal aniline hydroxylation is not enhanced by haeme, ferric ion or albumin. 4 Catalase and cyanide ions are powerful inhibitors of haemoglobin-mediated aniline hydroxylation both in the presence and absence of tissue. Carbon monoxide inhibits the hydroxylase activity of the tissue-free system to a smaller extent than that of a system containing microsomes plus haemoglobin whereas p-chloromercuribenzoate inhibits only the flavoprotein-dependent hydroxylation of aniline mediated by haemoglobin. 5. Several possibilities of interactions between substrate, microsomes and haeme compounds are proposed.

摘要

血红蛋白和肌红蛋白可增强大鼠肝微粒体对苯胺和乙酰苯胺的对羟基化作用。血红素蛋白不会增加乙基吗啡和氨基比林的微粒体N-去甲基化作用。2. 在高底物浓度和高血红素化合物浓度下，微粒体对羟基化作用的增强最为显著。3. 经去污剂纯化的NADPH-细胞色素c还原酶、游离黄素和锰离子可显著增强血红蛋白介导的、无组织情况下苯胺的羟基化作用。血红素、铁离子或白蛋白不会增强微粒体苯胺羟基化作用。4. 无论有无组织存在，过氧化氢酶和氰离子都是血红蛋白介导的苯胺羟基化作用的强力抑制剂。一氧化碳对无组织系统羟化酶活性的抑制程度小于对含有微粒体加血红蛋白系统的抑制程度，而对氯汞苯甲酸仅抑制血红蛋白介导的依赖黄素蛋白的苯胺羟基化作用。5. 提出了底物、微粒体和血红素化合物之间相互作用的几种可能性。