血红蛋白对微粒体苯胺和乙酰苯胺羟化作用的增强
Enhancement of microsomal aniline and acetanilide hydroxylation by haemoglobin.
作者信息
Jonen H G, Kahl R, Kahl G F
出版信息
Xenobiotica. 1976 May;6(5):307-20. doi: 10.3109/00498257609151642.
PMID:820088
Abstract
- Haemogloblin and myoglobin enhance rat liver microsomal p-hydroxylation of aniline and acetanilide. Microsomal N-demethylation of ethylmorphine and aminopyrine is not increased by haemoproteins. 2. The enhancement of microsomal p-hydroxylation is maximal at high substrate concentration and high haeme compound concentration. 3. Detergent-purified NADPH-cytochrome c reductase, free flavins and manganese ions considerably increase the haemoglobin-mediated, tissue-free hydroxylation of aniline. Microsomal aniline hydroxylation is not enhanced by haeme, ferric ion or albumin. 4 Catalase and cyanide ions are powerful inhibitors of haemoglobin-mediated aniline hydroxylation both in the presence and absence of tissue. Carbon monoxide inhibits the hydroxylase activity of the tissue-free system to a smaller extent than that of a system containing microsomes plus haemoglobin whereas p-chloromercuribenzoate inhibits only the flavoprotein-dependent hydroxylation of aniline mediated by haemoglobin. 5. Several possibilities of interactions between substrate, microsomes and haeme compounds are proposed.
摘要
- 血红蛋白和肌红蛋白可增强大鼠肝微粒体对苯胺和乙酰苯胺的对羟基化作用。血红素蛋白不会增加乙基吗啡和氨基比林的微粒体N-去甲基化作用。2. 在高底物浓度和高血红素化合物浓度下，微粒体对羟基化作用的增强最为显著。3. 经去污剂纯化的NADPH-细胞色素c还原酶、游离黄素和锰离子可显著增强血红蛋白介导的、无组织情况下苯胺的羟基化作用。血红素、铁离子或白蛋白不会增强微粒体苯胺羟基化作用。4. 无论有无组织存在，过氧化氢酶和氰离子都是血红蛋白介导的苯胺羟基化作用的强力抑制剂。一氧化碳对无组织系统羟化酶活性的抑制程度小于对含有微粒体加血红蛋白系统的抑制程度，而对氯汞苯甲酸仅抑制血红蛋白介导的依赖黄素蛋白的苯胺羟基化作用。5. 提出了底物、微粒体和血红素化合物之间相互作用的几种可能性。
相似文献
1
Enhancement of microsomal aniline and acetanilide hydroxylation by haemoglobin.血红蛋白对微粒体苯胺和乙酰苯胺羟化作用的增强
Xenobiotica. 1976 May;6(5):307-20. doi: 10.3109/00498257609151642.
2
Influence of the oral contraceptive, menstranol, on drug-metabolizing enzymes of female rats in thiamin-supplemented and deficiency states.口服避孕药炔雌醇甲醚对硫胺素补充和缺乏状态下雌性大鼠药物代谢酶的影响。
Pharmacology. 1976;14(2):104-14. doi: 10.1159/000136586.
3
Effects of 6-aminochrysene on liver microsomal enzyme activity.6-氨基 Chrysene 对肝微粒体酶活性的影响。
Xenobiotica. 1976 Apr;6(4):201-5. doi: 10.3109/00498257609151630.
4
The effect of certain vitamin deficiencies on hepatic drug metabolism.某些维生素缺乏对肝脏药物代谢的影响。
Fed Proc. 1976 Nov;35(13):2464-9.
5
Cytochrome P450 mediated drug metabolism in the streptozotocin diabetic rat.细胞色素P450介导的链脲佐菌素糖尿病大鼠体内的药物代谢
Horm Metab Res. 1980 Dec;12(12):706-7. doi: 10.1055/s-2007-999239.
6
Kinetics of ethylmorphine N-demethylase at low substrate concentrations and its implications concerning the role of the stimulation of NADPH-cytochrome P-450 reductase activity in mixed-function oxidase activity.低底物浓度下乙基吗啡N-脱甲基酶的动力学及其与NADPH-细胞色素P-450还原酶活性刺激在混合功能氧化酶活性中作用的关系。
Biochem Pharmacol. 1977 Oct 1;26(19):1803-7. doi: 10.1016/0006-2952(77)90349-5.
7
Development of liver microsomal oxidations in the chick.雏鸡肝脏微粒体氧化作用的发育
Xenobiotica. 1976 Feb;6(2):69-81. doi: 10.3109/00498257609151616.
8
Contrasting effect of isoflurane on drug metabolism: decreased type I and increased type II substrate metabolism in guinea pig liver microsomes.异氟烷对药物代谢的对比效应：豚鼠肝微粒体中I型底物代谢降低，II型底物代谢增加。
J Appl Toxicol. 1996 Jul-Aug;16(4):331-7. doi: 10.1002/(SICI)1099-1263(199607)16:4<331::AID-JAT354>3.0.CO;2-L.
9
Differential inhibition of drug metabolism by hepatic microsomal lipids of neonatal and adult rats.新生大鼠和成年大鼠肝脏微粒体脂质对药物代谢的差异抑制作用。
Biochem Biophys Res Commun. 1975 Aug 4;65(3):870-6. doi: 10.1016/s0006-291x(75)80466-9.
10
The role of NADPH-cytochrome c reductase in microsomal hydroxylation reactions.NADPH-细胞色素c还原酶在微粒体羟化反应中的作用。
Arch Biochem Biophys. 1975 Sep;170(1):160-8. doi: 10.1016/0003-9861(75)90107-1.
Zotero 插件