Shimada K, Mitamura K, Kaji H, Morita M
Faculty of Pharmaceutical Sciences, Kanazawa University, Japan.
J Chromatogr Sci. 1994 Mar;32(3):107-11. doi: 10.1093/chromsci/32.3.107.
The retention behavior of sulfates or glucuronides of provitamin D, vitamin D, and 25-hydroxyvitamin D3, together with its fluorescent derivatives, are examined with reversed-phase high-performance liquid chromatography. Inclusion chromatography using cyclodextrin as a mobile-phase additive is also used for this purpose. Conventional reversed-phase high-performance liquid chromatography clearly separates the positionally isomeric conjugates of 25-hydroxyvitamin D3. The addition of the host compound to the mobile phase is effective in separating the pairs of fluorescent derivatives of vitamin-D3 and -D2 conjugates or provitamin-D3 and -D2 conjugates.
采用反相高效液相色谱法研究了维生素原D、维生素D和25-羟基维生素D3的硫酸盐或葡糖醛酸苷及其荧光衍生物的保留行为。为此也使用了以环糊精作为流动相添加剂的包合色谱法。传统的反相高效液相色谱法能清晰分离25-羟基维生素D3的位置异构体结合物。在流动相中添加主体化合物可有效分离维生素D3和D2结合物或维生素原D3和D2结合物的荧光衍生物对。
