Matheis G, Belitz H D
Z Lebensm Unters Forsch. 1975 Apr 7;157(4):221-7. doi: 10.1007/BF01785774.
Upon polyacrylamide gel electrophoresis, a soluble phenoloxidase from potatoes (var. Maritta) revealed 17 multiple forms with activity towards dopa and almost all other o-diphenols tested, but only 5 of the forms reacted with monophenols. Isoelectric focusing of the crude enzyme resulted in 2 main peaks with activity towards dopa, having isoelectric points at pH ranges 4.0-4.7 and 5.1-5.4: smaller amounts of the enzyme at higher pI values were also detected. When activity peaks were controlled by polyacrylamide gel electrophoresis, all bands previously detected by electrophoresis of the crude enzyme were recovered, but all peaks were electrophoretically heterogeneous. Gel chromatography of the crude enzyme showed different molecular forms. Their molecular weights indicated monomer, dimer, tetramer, octamer and polymer (at least hexadecamer) forms with a monomer molecular weight of about 36000.
在聚丙烯酰胺凝胶电泳中，来自马铃薯（品种Maritta）的一种可溶性酚氧化酶显示出17种对多巴以及几乎所有测试的其他邻二酚具有活性的多种形式，但只有5种形式与单酚发生反应。粗酶的等电聚焦产生了2个对多巴具有活性的主要峰，其等电点在pH范围4.0 - 4.7和5.1 - 5.4：也检测到较高pI值下较少量的酶。当通过聚丙烯酰胺凝胶电泳控制活性峰时，回收了先前通过粗酶电泳检测到的所有条带，但所有峰在电泳上都是异质的。粗酶的凝胶色谱显示出不同的分子形式。它们的分子量表明存在单体、二聚体、四聚体、八聚体和聚合物（至少十六聚体）形式，单体分子量约为36000。
