通过X射线纤维衍射确定黄瓜绿斑驳花叶病毒的结构。对烟草花叶病毒进化的意义。
Structure determination of cucumber green mottle mosaic virus by X-ray fiber diffraction. Significance for the evolution of tobamoviruses.
作者信息
Wang H, Stubbs G
机构信息
Department of Molecular Biology, Vanderbilt University, Nashville, TN 37235.
出版信息
J Mol Biol. 1994 Jun 10;239(3):371-84. doi: 10.1006/jmbi.1994.1379.
Cucumber green mottle mosaic virus (CGMMV) is a rod-shaped virus of the tobacco mosaic virus (TMV) group. The structure of cucumber green mottle mosaic virus has been determined by fiber diffraction methods at 3.4 A resolution, and refined by molecular dynamics methods to an R factor of 0.093. Disassembly of TMV is driven by the mutual repulsion of intersubunit carboxyl-carboxylate pairs, but one of these pairs is not conserved in CGMMV. An alternative pair, located about 5 A from the site of the TMV pair, has been found in CGMMV. Comparison of the two structures suggests that the carboxylate groups are free to migrate in the subunit interfaces during evolution.
黄瓜绿斑驳花叶病毒（CGMMV）是烟草花叶病毒（TMV）组的一种杆状病毒。黄瓜绿斑驳花叶病毒的结构已通过纤维衍射方法在3.4埃分辨率下确定，并通过分子动力学方法精修至R因子为0.093。TMV的解体是由亚基间羧基-羧酸盐对的相互排斥驱动的，但其中一对在CGMMV中并不保守。在CGMMV中发现了另一对位于距TMV对位点约5埃处的（羧基-羧酸盐对）。两种结构的比较表明，在进化过程中，羧酸盐基团在亚基界面中可自由迁移。
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