Nativ O, Medalia O, Engelberg S, Raviv G, Aronson M
Tel Hashomer Hospital, Tel Aviv, Israel.
J Urol. 1994 Jul;152(1):217-9. doi: 10.1016/s0022-5347(17)32865-3.
Previous studies from our laboratory have shown that some Escherichia coli endotoxins are capable of inducing massive normal urothelial cell shedding. In the present study we investigated whether endotoxin-induced shedding improves cytologic detection of early stage mouse bladder cancer. Mouse bladder tumor (MBT-2) cells were implanted intravesically in the submucosa of C3H female mice. Ten to 21 days later the bladders were irrigated with saline followed by instillation of endotoxin. The bladder contents of each mouse were aspirated and examined cytologically together with the bladder wash specimen. Shedding of epithelial cells was observed in only 32% of the saline irrigated specimens compared with 93% after endotoxin instillation (p < 0.00001). Analysis showed an overall accuracy rate of 39% after saline barbotage versus 78% following endotoxin administration (p < 0.00001). These results indicate that intravesical instillation of specific bacterial endotoxin significantly increases the extent of cytologic detection of early stage superficial bladder cancer.
我们实验室之前的研究表明，一些大肠杆菌内毒素能够诱导大量正常尿路上皮细胞脱落。在本研究中，我们调查了内毒素诱导的细胞脱落是否能改善早期小鼠膀胱癌的细胞学检测。将小鼠膀胱肿瘤（MBT-2）细胞经膀胱内植入C3H雌性小鼠的黏膜下层。10至21天后，用盐水冲洗膀胱，随后注入内毒素。抽吸每只小鼠的膀胱内容物，并与膀胱冲洗标本一起进行细胞学检查。与注入内毒素后93%的标本相比，仅32%的盐水冲洗标本观察到上皮细胞脱落（p<0.00001）。分析显示，盐水灌洗后的总体准确率为39%，而给予内毒素后的准确率为78%（p<0.00001）。这些结果表明，膀胱内注入特定细菌内毒素可显著提高早期浅表性膀胱癌的细胞学检测范围。
