用琼脂凝胶扩散和免疫电泳技术鉴别布万巴病毒和庞戈拉病毒。

Differentiation of Bwamba and Pongola viruses by agar-gel diffusion and immunoelectrophoretic techniques.

作者信息

Tomori O, Fabiyi A

出版信息

Am J Trop Med Hyg. 1976 May;25(3):489-93. doi: 10.4269/ajtmh.1976.25.489.

DOI:10.4269/ajtmh.1976.25.489

PMID:820211

Abstract

The agar-gel diffusion (AGD) and the immunoelectrophoretic (IEP) techniques were employed in the differentiation of virus strains of the Bwamba-Pongola group. These techniques were found to be more specific than either the complement fixation (CF) or the neutralization (N) test in the differentiation of Bwamba and Pongola viurses. Of 22 virus strains isolated in Nigeria and previously typed as strains of Bwamba virus by CF and N tests, 12 strains of mosquito origin were definitely classified as Pongola viruses and 10 of human origin as Bwamba viruses by the AGD and IEP techniques.

摘要

采用琼脂凝胶扩散（AGD）和免疫电泳（IEP）技术对布万巴-蓬戈拉病毒组的病毒株进行鉴别。结果发现，在鉴别布万巴病毒和蓬戈拉病毒方面，这些技术比补体结合（CF）试验或中和（N）试验更具特异性。在尼日利亚分离出的22株病毒，此前通过CF试验和N试验鉴定为布万巴病毒株，通过AGD和IEP技术鉴定，其中12株源自蚊子的病毒株被明确归类为蓬戈拉病毒，10株源自人类的病毒株为布万巴病毒。