Rao M G, Butchaiah G, Sen A K
Indian Veterinary Research Institute Campus, Hebbal, Bangalore.
Vet Microbiol. 1994 Mar;39(1-2):135-43. doi: 10.1016/0378-1135(94)90094-9.
The antibody response to foot-and-mouth disease virus (FMDV) antigens of type Asia-1 in guinea-pigs was studied by micro-serum neutralization test (MSNT) and enzyme-linked immunosorbent assay (ELISA). One inoculation of as little as 1 microgram of binary ethyleneimine (BEI)-inactivated 146S virus particles in guinea-pigs elicited enough neutralizing antibodies to protect them against challenge with virulent virus. However, one inoculation of live 146S virus particles elicited higher levels of neutralizing antibodies in guinea-pigs than that of inactivated 146S particles. One inoculation of 12S protein subunits in guinea-pigs elicited only non-neutralizing anti-12S antibodies detected by ELISA. Similarly, non-neutralizing anti-VP1 antibodies were detected by ELISA after one inoculation of VP1. However, multiple inoculations of 12S or VP1 elicited measurable neutralizing antibodies in guinea-pigs that protected them against challenge.
通过微量血清中和试验（MSNT）和酶联免疫吸附测定（ELISA）研究了豚鼠对亚洲1型口蹄疫病毒（FMDV）抗原的抗体反应。在豚鼠中单次接种低至1微克的双乙烯亚胺（BEI）灭活的146S病毒颗粒可引发足够的中和抗体，以保护它们免受强毒病毒的攻击。然而，单次接种活的146S病毒颗粒在豚鼠中引发的中和抗体水平高于灭活的146S颗粒。在豚鼠中单次接种12S蛋白亚基仅引发ELISA检测到的非中和性抗12S抗体。同样，单次接种VP1后通过ELISA检测到非中和性抗VP1抗体。然而，多次接种12S或VP1可在豚鼠中引发可测量的中和抗体，从而保护它们免受攻击。
