亚洲1型口蹄疫病毒单克隆抗体的制备与鉴定
Preparation and characterization of monoclonal antibodies to foot-and-mouth disease virus type Asia-1.
作者信息
Amineva S P, Davydova N L, Mamatkulova L U, Globenko L A, Mishchenko V A
机构信息
Institute of Animal Health, Russian Ministry of Agriculture and Food, Vladimir Region, Russia.
出版信息
Biochemistry (Mosc). 1997 May;62(5):469-73.
Eight clones of hybrid cells secreting monoclonal antibodies to foot-and-mouth disease virus type Asia-1 were prepared; three of them neutralized viral infection. The specificity of THE monoclonal antibodies was analyzed by various immunoenzyme assays using 146S viral particles, trypsin-treated 146S particles, 12S particles, and certain viral polypeptides. The epitopes unique for virus type Asia-1 and conservative among several types were detected on the surface of viral particles. Epitopes on the surface of viral particle were mapped.
制备了8个分泌抗亚洲1型口蹄疫病毒单克隆抗体的杂交细胞克隆；其中3个可中和病毒感染。使用146S病毒颗粒、胰蛋白酶处理的146S颗粒、12S颗粒和某些病毒多肽，通过各种免疫酶测定法分析了单克隆抗体的特异性。在病毒颗粒表面检测到亚洲1型病毒特有的且在几种类型中保守的表位。绘制了病毒颗粒表面的表位图谱。
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