Sur I P, Lobo Z, Maitra P K
Molecular Biology Unit, Tata Institute of Fundamental Research, Colaba, Bombay, India.
Yeast. 1994 Feb;10(2):199-209. doi: 10.1002/yea.320100207.
The pfk3 mutation of Saccharomyces cerevisiae causes glucose-negativity in a pfk1 genetic background, the mutant is temperature-sensitive for growth and homozygous diploids do not sporulate. It fails to accumulate trehalose, and has an altered glycogen accumulation profile under glucose-starvation conditions. pfk3-6, one of the alleles of pfk3, has an altered morphology, forming long chain-like structures at 36 degrees C. The PFK3 gene was cloned by complementation of the mutant phenotypes. Integrative transformation demonstrated that the complementing fragment encoded the authentic PFK3 gene. The disruption of the gene does not affect viability. Like the EMS-induced pfk3 mutant, the disruptants are temperature-sensitive and in a pfk1 genetic background are also glucose-negative. The PFK3 transcript is induced by heat-shock. Partial DNA sequence shows that PFK3 is identical to TPS2 (De Virgilio et al., 1993). We demonstrate that, apart from being a structural determinant of trehalose 6-phosphate phosphatase, PFK3 (TPS2) is required for PFKII synthesis and normal regulation of S. cerevisiae response to nutrient and thermal stresses.
酿酒酵母的pfk3突变在pfk1基因背景下导致葡萄糖阴性，该突变体对生长具有温度敏感性，纯合二倍体不产孢。它无法积累海藻糖，并且在葡萄糖饥饿条件下糖原积累模式发生改变。pfk3的等位基因之一pfk3-6具有改变的形态，在36℃时形成长链状结构。通过对突变体表型的互补作用克隆了PFK3基因。整合转化表明互补片段编码了真实的PFK3基因。该基因的破坏不影响生存能力。与EMS诱导的pfk3突变体一样，破坏株对温度敏感，在pfk1基因背景下也是葡萄糖阴性。PFK3转录本受热激诱导。部分DNA序列表明PFK3与TPS2相同（德·维尔吉利奥等人，1993年）。我们证明，除了作为海藻糖6-磷酸磷酸酶的结构决定因素外，PFK3（TPS2）对于PFKII的合成以及酿酒酵母对营养和热应激反应的正常调节是必需的。
