Terada N, Konno A, Fukuda S, Yamashita T, Shirotori K, Okamoto Y, Ishikawa K, Togawa K
Department of Otorhinolaryngology, Akita University School of Medicine, Japan.
Acta Otolaryngol. 1994 Mar;114(2):203-8. doi: 10.3109/00016489409126043.
Eosinophils and their products are known to cause hyperreactivity and swelling of the nasal mucosa in subjects with nasal allergy. Interleukin-5 (IL-5) not only induces differentiation and proliferation of immature eosinophils but also causes mature cells to accumulate and activate. This study shows that IL-5 is actually produced in the human nasal mucosa by antigen challenge, and it further investigates the changes in the amount of IL-5 in nasal lavage fluids after antigen challenge. Expression of mRNA for IL-5 in nasal mucosa was investigated using the reverse transcription-polymerase chain reaction (RT-PCR) and Southern blot analysis. Among the 4 subjects with nasal allergy examined in this study, expression of mRNA for IL-5 was observed in 2 prior to antigen challenge; within 6 h after antigen challenge it was seen in 3 subjects. We also found that the amount of IL-5 in the nasal lavage fluids obtained consecutively after antigen challenge increased predominantly in the late phase, and that the number of eosinophils in the IL-5 positive group was significantly higher than that in the IL-5 negative group. These results strongly suggest that IL-5 contributes to the recruitment of eosinophils in the nasal mucosa of the subjects with nasal allergy.
已知嗜酸性粒细胞及其产物会导致鼻过敏患者的鼻黏膜出现高反应性和肿胀。白细胞介素-5（IL-5）不仅能诱导未成熟嗜酸性粒细胞的分化和增殖，还会使成熟细胞聚集并激活。本研究表明，抗原激发后，人鼻黏膜中确实会产生IL-5，并进一步研究了抗原激发后鼻灌洗液中IL-5含量的变化。使用逆转录-聚合酶链反应（RT-PCR）和Southern印迹分析研究了鼻黏膜中IL-5的mRNA表达。在本研究检测的4名鼻过敏患者中，2名在抗原激发前观察到IL-5的mRNA表达；抗原激发后6小时内，3名患者出现了该表达。我们还发现，抗原激发后连续获取的鼻灌洗液中IL-5的含量在后期主要增加，且IL-5阳性组中的嗜酸性粒细胞数量明显高于IL-5阴性组。这些结果有力地表明，IL-5有助于鼻过敏患者鼻黏膜中嗜酸性粒细胞的募集。
