Mathew A, Grdisa M, Robbins P J, White M K, Johnstone R M
Department of Biochemistry, McGill University, Montreal, Quebec, Canada.
Am J Physiol. 1994 May;266(5 Pt 1):C1222-30. doi: 10.1152/ajpcell.1994.266.5.C1222.
The HD3 cell, a chicken erythroblast cell line infected with a temperature-sensitive avian erythroblastosis virus, becomes committed to differentiate to an erythrocyte upon temperature shift in presence of inducers. Before induction, the HD3 cell transports glucose and 2-deoxyglucose (2-DG). 3-O-methylglucose is poorly taken up. Upon induction of differentiation, glucose and 2-DG transport activity fall. Twenty-four hours postinduction, up to 75% of the glucose transport activity may disappear. By use of cDNA probes for chicken glucose transporters, two species of mRNA of 3.1 and 1.7 kb (equivalent to mammalian GLUT1 and GLUT3 mRNA, respectively) are detected. Both messages virtually disappear within 48 h after induction. Run-on assays show the cessation of synthesis of the corresponding RNAs parallel to the loss of glucose transport. In contrast to the glucose transporters, the nucleoside transporter level increases after induction of hematopoiesis. This developmental pattern is consistent with earlier studies showing that mature chicken erythrocytes have little glucose transport activity but retain appreciable levels of the nucleoside transporter and that nucleosides and glutamine provide major sources of oxidizable carbon compounds to sustain metabolism in circulating chicken erythrocytes.
HD3细胞是一种感染了温度敏感型禽成红细胞增多症病毒的鸡成红细胞系，在诱导剂存在的情况下，温度转变时会定向分化为红细胞。诱导前，HD3细胞可转运葡萄糖和2-脱氧葡萄糖（2-DG）。3-O-甲基葡萄糖摄取能力较差。诱导分化后，葡萄糖和2-DG转运活性下降。诱导后24小时，高达75%的葡萄糖转运活性可能消失。通过使用鸡葡萄糖转运体的cDNA探针，检测到两种分别为3.1 kb和1.7 kb的mRNA（分别相当于哺乳动物的GLUT1和GLUT3 mRNA）。诱导后48小时内，这两种信使RNA几乎都消失了。连续转录分析表明，相应RNA合成的停止与葡萄糖转运的丧失同步。与葡萄糖转运体不同，造血诱导后核苷转运体水平升高。这种发育模式与早期研究一致，早期研究表明成熟的鸡红细胞葡萄糖转运活性很低，但保留了相当水平的核苷转运体，并且核苷和谷氨酰胺是循环中的鸡红细胞维持代谢的主要可氧化碳化合物来源。
