Lee W J, Brey P T
Unité d'Ecologie des Systèmes Vectoriels, Institut Pasteur, Paris, France.
Anal Biochem. 1994 Mar;217(2):231-5. doi: 10.1006/abio.1994.1113.
A method for the screening and isolation of antibacterial peptides from the extracellular insect cuticle using ultrafiltration and reverse-phase high-performance liquid chromatography is presented. Using this method we were able to conveniently isolate cuticular antibacterial peptides belonging to the cecropin family in 1 day. The procedure should facilitate the rapid screening of antibacterial peptides or other bioactive peptides from extracellular matrices.
本文介绍了一种利用超滤和反相高效液相色谱从昆虫细胞外角质层中筛选和分离抗菌肽的方法。使用该方法，我们能够在1天内方便地分离出属于天蚕素家族的角质层抗菌肽。该方法应有助于从细胞外基质中快速筛选抗菌肽或其他生物活性肽。
