1,25-二羟基维生素D3和视黄酸对神经母细胞瘤SK-N-SH细胞增殖及细胞周期阶段分布的影响

Effects of 1,25-dihydroxyvitamin D3 and retinoic acid on the proliferation and cell cycle phase distribution of neuroblastoma SK-N-SH cells.

作者信息

Goplen D P, Brackman D, Aksnes L

机构信息

Institute of Pediatrics, University of Bergen, Norway.

出版信息

Pediatr Hematol Oncol. 1994 Mar-Apr;11(2):173-9. doi: 10.3109/08880019409141653.

DOI:10.3109/08880019409141653

PMID:8204442

Abstract

The hormone 1,25-(OH)2D3 has been shown to modulate cell proliferation and induce differentiation in several normal and malignant cell lines. In this work, we examined the effect of the hormone on the neuroblastoma SK-N-SH cell line. The steroid did not influence cell growth and cell cycle distribution, while retinoic acid inhibited proliferation and induced an accumulation of the cells in the G0/G1 phase of the cell cycle. 1,25-(OH)2D3 did not alter cell morphology. The activities of the 1-alpha- and 24-hydroxylases were low and not regulated by the hormone. The level of the total 1,25-(OH)2D3 receptor was low. We conclude that the lack of effect of 1,25-(OH)2D3 on the SK-N-SH cell line is related to the low level of the 1,25-(OH)2D3 receptor.

摘要

已证明激素1,25 -（OH）2D3可调节几种正常和恶性细胞系中的细胞增殖并诱导分化。在这项研究中，我们检测了该激素对神经母细胞瘤SK - N - SH细胞系的影响。该类固醇对细胞生长和细胞周期分布没有影响，而视黄酸则抑制增殖并诱导细胞在细胞周期的G0/G1期积累。1,25 -（OH）2D3没有改变细胞形态。1-α-羟化酶和24-羟化酶的活性较低，且不受该激素调节。1,25 -（OH）2D3受体的总水平较低。我们得出结论，1,25 -（OH）2D3对SK - N - SH细胞系缺乏作用与1,25 -（OH）2D3受体水平较低有关。