Hsieh Y L, Li Y T, Henion J D, Ganem B
Diagnostic Laboratory, New York State College of Veterinary Medicine, Cornell University, Ithaca 14850.
Biol Mass Spectrom. 1994 May;23(5):272-6. doi: 10.1002/bms.1200230506.
Non-covalent binding of actinomycin D (AMD) to single-stranded DNA (ssDNA) was observed by ion spray mass spectrometry. Interactions between AMD and different sequences of non-self-complementary oligodeoxynucleotides were investigated as a function of pH. Non-covalent AMD/ssDNA complexes disappeared at low pH, and no ssDNA complexes were detected with rifampicin (RP). Moreover, the different binding affinities between AMD and ssDNA with and without 5'G-C3' or 5'C-G3' base sequences were also demonstrated using ion spray mass spectrometry. Additional support for the non-covalent nature of binding in AMD/ssDNA complexes was obtained from tandem mass spectrometry.
通过离子喷雾质谱法观察到放线菌素D（AMD）与单链DNA（ssDNA）的非共价结合。研究了AMD与不同序列的非自互补寡脱氧核苷酸之间的相互作用随pH的变化。非共价AMD/ssDNA复合物在低pH下消失，并且未检测到利福平（RP）与ssDNA的复合物。此外，还使用离子喷雾质谱法证明了AMD与具有或不具有5'G-C3'或5'C-G3'碱基序列的ssDNA之间不同的结合亲和力。串联质谱法为AMD/ssDNA复合物中结合的非共价性质提供了额外的支持。
