大鼠肾脏组织切片中Ⅰ型胶原的免疫组织化学微量定量方法。

Immunohistochemical microquantitation method for type I collagen in kidney histological section of the rats.

作者信息

Manabe N, Furuya Y, Nagano N, Miyamoto H

机构信息

Department of Animal Science, Faculty of Agriculture, Kyoto University, Japan.

出版信息

J Vet Med Sci. 1994 Feb;56(1):147-50. doi: 10.1292/jvms.56.147.

DOI:10.1292/jvms.56.147

PMID:8204741

Abstract

An immunohistochemical micromethod for quantitation of type I collagen in frozen histological section of rat kidney was developed. A principle of this method is enzyme-linked immunosorbent assay (ELISA). The intensity of immunoreactivity of the section to an anti-type I collagen antibody was quantitated, and then the total protein content in the same section was measured colorimetrically. Type I collagen content was expressed as mg of type I collagen per g of total protein. In this micromethod, the minimum area and optimum thickness of the section were 5 mm2 and 10 microns, respectively. Type I collagen content in the rat kidney section was 3.02 +/- 0.12 mg/g. This method should be useful for diagnosis of renal failure.

摘要

建立了一种用于定量大鼠肾脏冷冻组织切片中I型胶原蛋白的免疫组织化学微量方法。该方法的原理是酶联免疫吸附测定（ELISA）。对切片与抗I型胶原蛋白抗体的免疫反应强度进行定量，然后用比色法测量同一切片中的总蛋白含量。I型胶原蛋白含量以每克总蛋白中I型胶原蛋白的毫克数表示。在这种微量方法中，切片的最小面积和最佳厚度分别为5平方毫米和10微米。大鼠肾脏切片中I型胶原蛋白含量为3.02±0.12毫克/克。该方法应有助于肾衰竭的诊断。