Malkusch W, Rehn B, Bruch J
Institute of Hygiene and Occupational Medicine, University Hospital, Gesamthochschule Essen, Germany.
Exp Lung Res. 1995 Jan-Feb;21(1):67-77. doi: 10.3109/01902149509031745.
Sirius Red staining is presented as a method for collagen determination, enabling quantitative morphometric measurements to be performed in locally defined tissue areas. The advantage of this method is especially shown for alveolar lung tissue. By excluding the bronchial areas in the tissue sections, the differences in the degree of fibrosis proved to be more discrete after different loads of quartz dust than by any other method. The difference of 12 micrograms collagen measured colorimetrically represented a 1.2-fold increase. The collagen measured in the alveolar tissue by the morphometric method rose from 9.8 to 28.6%. This is a 2.9-fold increase, underlining the vast improvement in sensitivity. Thus, this method is specifically suitable for the evaluation of very small fibrotic lesions. The quartz doses given are particularly low compared to most other investigations. Histologic lung and lymph node sections from female Wistar rats injected intratracheally with differing quantities of quartz dust (0.03, 0.1, 0.5, 1.75 mg) were stained with Sirius Red, and the collagen fibers measured with a quantitative image analysis. The results for lymph nodes using different methods (wet weight determination, quantitative measurement of quartz typical areas, colorimetric and morphometric collagen determination) showed a high correlation at the different doses. This showed that the morphometric method is suitable for the quantitative measurement of collagen. Corresponding results were also found in the comparative lung tissue measurements (colorimetric and morphometric collagen determination). However, the morphometric method has the decisive advantage that measurements can be restricted to defined tissue areas and do not destroy the section.
天狼星红染色是一种用于测定胶原蛋白的方法，可在局部限定的组织区域进行定量形态测量。这种方法的优势在肺泡肺组织中尤为明显。通过在组织切片中排除支气管区域，在不同剂量的石英粉尘作用后，纤维化程度的差异比其他任何方法都更离散。比色法测得的12微克胶原蛋白差异代表增加了1.2倍。形态测量法测得的肺泡组织中的胶原蛋白从9.8%升至28.6%。这是2.9倍的增长，突出了敏感性的大幅提高。因此，该方法特别适用于评估非常小的纤维化病变。与大多数其他研究相比，所给予的石英剂量特别低。对经气管内注射不同量石英粉尘（0.03、0.1、0.5、1.75毫克）的雌性Wistar大鼠的肺和淋巴结组织切片进行天狼星红染色，并用定量图像分析测量胶原纤维。使用不同方法（湿重测定、石英典型区域的定量测量、比色法和形态测量法测定胶原蛋白）对淋巴结的结果显示，在不同剂量下具有高度相关性。这表明形态测量法适用于胶原蛋白的定量测量。在肺组织的对比测量（比色法和形态测量法测定胶原蛋白）中也发现了相应的结果。然而，形态测量法具有决定性的优势，即测量可以局限于限定的组织区域，并且不会破坏切片。
