从采血纸上采集的全血中检测抗猪巨细胞病毒抗体。
Detection of the antibodies against porcine cytomegalovirus from whole blood collected on the blood sampling paper.
作者信息
Tajima T, Hironao T, Kajikawa T, Suzuki Y, Kawamura H
机构信息
Department of Veterinary Microbiology, College of Agriculture, University of Osaka Prefecture, Japan.
出版信息
J Vet Med Sci. 1994 Feb;56(1):189-90. doi: 10.1292/jvms.56.189.
The usefulness of blood sampling paper for the detection of antibodies against porcine cytomegalovirus (PCMV) by enzyme-linked immunosorbent assay (ELISA) was examined. The PCMV antibody titer of whole blood collected on the paper agreed with that of serum and the absorbances of the corresponding samples were well correlated (r = 0.88). Blood was collected from 26 piglets in the field on the blood sampling paper and ELISA was performed. The maternal PCMV antibody of these piglets decreased during the 8 weeks after birth and IgM antibody against PCMV was detected in four piglets.
检测了采血滤纸用于通过酶联免疫吸附测定（ELISA）检测抗猪巨细胞病毒（PCMV）抗体的实用性。采自滤纸上的全血的PCMV抗体效价与血清的一致，且相应样本的吸光度具有良好的相关性（r = 0.88）。在野外从26头仔猪采集血样于采血滤纸上并进行ELISA检测。这些仔猪的母源PCMV抗体在出生后8周内下降，并且在4头仔猪中检测到抗PCMV的IgM抗体。
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