[Capturing-ELISA for the detection of anti-HCMV-IgM antibody in serum].
作者信息
Zhang Xin-ping, Li Pei, Liu Hong-jing, Liu Bao-gen
机构信息
Laboratory of Medical Technology, Hangzhou Sixth People's Hospital, Zhejiang Province, Hangzhou 310014, China.
出版信息
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2003 Sep;17(3):285-6.
BACKGROUND
To develop a capturing-ELISA for the detection of anti-HCMV-IgM antibody in serum.
METHODS
The anti-HCMV-IgM antibody was detected in 68 patients with HCMV infection by the capturing-ELISA, and the results were compared with those of indirect ELISA.
RESULTS
The specificity and sensitivity of the capturing-ELISA were shown to be significantly higher than those of indirect ELISA, and its results were not affected by RF factor.
CONCLUSION
The capturing ELISA is specific, sensitive, convenient and reliable method which may be feasible for clinical use.
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