一种用于蕨类植物材料快速固定和包埋的改进方法。
An improved method for rapid fixation and embedding of pteridophyte plant materials.
作者信息
Devi S, Lall V K, Garg P, Saxena A, Tiwari M
机构信息
Electron Microscopy Section, National Botanical Research Institute, Lucknow, India.
出版信息
Biotech Histochem. 1994 Mar;69(2):99-101. doi: 10.3109/10520299409106268.
PMID:8204772
Abstract
A rapid method for fixation and embedding of plant materials, especially pteridophytes, is suggested. Addition of tannic acid following osmication improved the visualization of membranes. Staining en bloc with uranyl acetate between osmication and tannic acid is suggested for tissues infected with fungi and bacteria.
摘要
本文提出了一种快速固定和包埋植物材料，特别是蕨类植物材料的方法。在锇酸固定后添加单宁酸可改善膜的可视化效果。对于感染真菌和细菌的组织，建议在锇酸固定和单宁酸处理之间用醋酸铀进行整体染色。
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