Taylor J E, Coupe S A, Picton S, Roberts J A
Department of Physiology and Environmental Science, Faculty of Agriculture and Food Sciences, University of Nottingham, Loughborough, Leics, UK.
Plant Mol Biol. 1994 Mar;24(6):961-4. doi: 10.1007/BF00014449.
A cDNA library was produced using mRNA extracted from ethylene-treated leaflet abscission zones of common elder (Sambucus nigra). Screening of the library with the insert from pBAC10, which encodes an abscission beta-1,4-glucanase (cellulase) from bean (Phaseolus vulgaris), resulted in the isolation of a near-full-length cDNA which was designated JET 1. Northern analysis, using JET 1 as a probe, detected a transcript of 1.9 kb that accumulated prior to the first visible signs of cell separation. Accumulation of the JET 1 transcript is promoted by ethylene and primarily restricted to the tissue comprising the abscission zone. Sequence analysis of JET 1 indicates it is 1768 bp in length and shares significant homology at the amino acid level with beta-1,4-glucanases from the leaf abscission zone of P. vulgaris (67%) and ripening avocado fruit (48%). The predicted peptide sequence of the S. nigra enzyme contains two potential glycosylation sites. Genomic Southern analysis of S. nigra DNA reveals that JET 1 may belong to a multi-gene family.
利用从经乙烯处理的接骨木（黑接骨木）小叶脱落区提取的mRNA构建了一个cDNA文库。用来自菜豆（菜豆）的编码脱落β-1,4-葡聚糖酶（纤维素酶）的pBAC10的插入片段筛选该文库，得到了一个几乎全长的cDNA，命名为JET 1。以JET 1为探针进行Northern分析，检测到一个1.9 kb的转录本，其在细胞分离的第一个可见迹象出现之前就开始积累。乙烯促进JET 1转录本的积累，且主要局限于包含脱落区的组织。JET 1的序列分析表明其长度为1768 bp，在氨基酸水平上与菜豆叶脱落区的β-1,4-葡聚糖酶（67%）和成熟鳄梨果实的β-1,4-葡聚糖酶（48%）具有显著同源性。黑接骨木酶的预测肽序列包含两个潜在的糖基化位点。黑接骨木DNA的基因组Southern分析表明JET 1可能属于一个多基因家族。
