Harris D T, Schumacher M J, Rychlik S, Booth A, Acevedo A, Rubinstein P, Bard J, Boyse E A
Department of Microbiology & Immunology, University of Arizona, Tucson 85721.
Bone Marrow Transplant. 1994 Feb;13(2):135-43.
Bone marrow transplantation (BMT) is limited by the paucity of HLA-matched donors and the frequent occurrence of graft-versus-host disease (GVHD). Recent clinical reports have implied that the use of umbilical cord blood (UCB) may alleviate some of the problems associated with BMT. Banks of frozen UCB could make the problem of finding suitable stem cell donors easier and stem cell grafts would be more readily available. However, definitive experiments are needed to develop optimal methods for collection, separation and storage of cryopreserved UCB for extended periods of time. We have found that several simple techniques may be utilized to collect large volumes of UCB (up to 220 ml). Also, modification of a common density gradient separation method permits recovery of large quantities of UCB mononuclear cells. Finally, we have examined the effects of prolonged frozen storage on the ability to recover viable and functional UCB, particularly stem/progenitor cells. It was observed that storage of UCB in liquid nitrogen for as long as 7 years had minimal effects on cell viability, cellular composition of UCB and progenitor/stem cell capacity. Thus, the establishment of UCB banks for use in transplantation appears to be a feasible approach.
骨髓移植（BMT）受到人类白细胞抗原（HLA）匹配供体稀缺以及移植物抗宿主病（GVHD）频繁发生的限制。近期临床报告表明，使用脐带血（UCB）可能会缓解一些与BMT相关的问题。冷冻脐带血库可以使寻找合适干细胞供体的问题变得更容易，并且干细胞移植物将更容易获得。然而，需要进行确定性实验来开发用于长时间收集、分离和储存冷冻保存的脐带血的最佳方法。我们发现可以利用几种简单技术来收集大量脐带血（高达220毫升）。此外，对一种常见密度梯度分离方法的改进能够回收大量脐带血单个核细胞。最后，我们研究了长期冷冻保存对恢复有活力且功能正常的脐带血，特别是干/祖细胞能力的影响。据观察，将脐带血在液氮中储存长达7年对细胞活力、脐带血的细胞组成以及祖细胞/干细胞能力的影响最小。因此，建立用于移植的脐带血库似乎是一种可行的方法。
