Kisi S, Yamaguchi M
Laboratory of Metabolism and Endocrinology, Graduate School of Nutritional Sciences, University of Shizuoka, Japan.
Biol Pharm Bull. 1994 Feb;17(2):345-7. doi: 10.1248/bpb.17.345.
The capability of beta-alanyl-L-histidinato zinc (AHZ) to increase alkaline phosphatase activity in the femoral diaphysis from elderly rats was investigated. The femoral-diaphyseal tissues were removed from weanling (3-week-old) and elderly (10-month-old) female rats. Bone tissues were cultured in Dulbecco's modified Eagle medium (high glucose, 4.5%) supplemented with antibiotics and bovine serum albumin. Among various other bone-stimulating factors (AHZ; 10(-5) M, zinc sulfate; 10(-4) M, sodium fluoride; 10(-3) M, insulin; 10(-8) M, and beta-estradiol; 10(-9) M), AHZ had a potent effect on increasing alkaline phosphatase activity in the diaphyseal tissues from both rat groups. In the bone tissues from elderly rats, the effect was concentration dependent (10(-7) - 10(-5) M). At 10(-5) M the effect of AHZ was seen for a longer time during 72-h culture, although the zinc sulfate (10(-5) M) effect was no longer. The effect of AHZ to increase bone alkaline phosphatase activity was completely abolished by the presence of cycloheximide (10(-6) M). AHZ thus appears able to directly stimulate alkaline phosphatase activity dependent on protein synthesis in the bone tissues from elderly rats.
研究了β-丙氨酰-L-组氨酸锌（AHZ）提高老年大鼠股骨干碱性磷酸酶活性的能力。从断奶（3周龄）和老年（10月龄）雌性大鼠中取出股骨干组织。将骨组织在补充有抗生素和牛血清白蛋白的杜氏改良 Eagle 培养基（高糖，4.5%）中培养。在各种其他骨刺激因子（AHZ；10⁻⁵ M、硫酸锌；10⁻⁴ M、氟化钠；10⁻³ M、胰岛素；10⁻⁸ M 和β-雌二醇；10⁻⁹ M）中，AHZ 对增加两组大鼠骨干组织中的碱性磷酸酶活性有显著作用。在老年大鼠的骨组织中，这种作用呈浓度依赖性（10⁻⁷ - 10⁻⁵ M）。在10⁻⁵ M 时，在72小时培养期间，AHZ 的作用持续时间更长，而硫酸锌（10⁻⁵ M）的作用则不再持续。放线菌酮（10⁻⁶ M）的存在完全消除了AHZ 增加骨碱性磷酸酶活性的作用。因此，AHZ 似乎能够直接刺激老年大鼠骨组织中依赖蛋白质合成的碱性磷酸酶活性。
