血浆中奎尼丁的自动固相萃取及高效液相色谱测定法
Automatic solid-phase extraction and high-performance liquid chromatographic determination of quinidine in plasma.
作者信息
Brandsteterová E, Romanová D, Králiková D, Bozeková L, Kriska M
机构信息
Department of Analytical Chemistry, Slovak Technical University, Bratislava.
出版信息
J Chromatogr A. 1994 Apr 8;665(1):101-4. doi: 10.1016/0021-9673(94)87036-5.
High-performance liquid chromatography (HPLC) was used for the therapeutic drug monitoring of quinidine in clinical samples. Solid-phase extraction (SPE) was studied in both off-line and on-line modes. SPE was performed in an automatic on-line mode using a fully automated Prospekt system. Extraction recoveries were in the range 97.1-99.4% for 1-2 micrograms/ml quinidine concentrations. For HPLC separation an Ultrasep RP-8 reversed-phase column was applied with acetonitrile-water (9:1) containing 0.3% triethylamine (pH 2.5) as the mobile phase. The Prospekt system is recommended for the routine monitoring of quinidine in plasma samples. Concentrations were in therapeutic range (1.2-3.6 micrograms/ml).
高效液相色谱法（HPLC）用于临床样本中奎尼丁的治疗药物监测。研究了离线和在线模式下的固相萃取（SPE）。使用全自动Prospekt系统以自动在线模式进行SPE。对于1-2微克/毫升的奎尼丁浓度，萃取回收率在97.1-99.4%范围内。HPLC分离采用Ultrasep RP-8反相柱，以含0.3%三乙胺（pH 2.5）的乙腈-水（9:1）作为流动相。推荐使用Prospekt系统对血浆样本中的奎尼丁进行常规监测。浓度处于治疗范围（1.2-3.6微克/毫升）。
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