采用C18固相萃取和基于多克隆抗体的免疫亲和柱纯化，通过等度高效液相色谱-库仑检测法测定生物样品中的催产素。
Determination of oxytocin in biological samples by isocratic high-performance liquid chromatography with coulometric detection using C18 solid-phase extraction and polyclonal antibody-based immunoaffinity column purification.
作者信息
Kukucka M A, Misra H P
机构信息
Department of Biomedical Sciences, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg 24061-0442.
出版信息
J Chromatogr B Biomed Appl. 1994 Mar 4;653(2):139-45. doi: 10.1016/0378-4347(93)e0414-l.
A specific high-performance liquid chromatographic (HPLC) method is described for the reliable quantitation of oxytocin using culture media supernatants. The procedure employs solid-phase extraction, antibody-based immunoaffinity purification and isocratic HPLC with dual channel coulometric detection (ED). The lower limit of detection for this cyclic nonapeptide was 40 pg (40 fmol). Due to its relative simplicity, specificity and precision, the HPLC-ED of oxytocin is an accurate and attractive alternative to many existing quantitative methods.
描述了一种使用培养基上清液可靠定量催产素的特定高效液相色谱（HPLC）方法。该程序采用固相萃取、基于抗体的免疫亲和纯化以及具有双通道库仑检测（ED）的等度HPLC。这种环状九肽的检测下限为40 pg（40 fmol）。由于其相对简单、特异性和精密度，催产素的HPLC-ED是许多现有定量方法的一种准确且有吸引力的替代方法。
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