Moltó L, Carballido J, Manzano L, Olivier C, Lapeña P, Alvarez-Mon M
Department of Medicine, Hospital Universitario Principe de Asturias, Universidad de Alcalá de Henares, Madrid, Spain.
Cancer Immunol Immunother. 1994 Jun;38(6):406-10. doi: 10.1007/BF01517211.
We have investigated the effect of interferon beta (INF beta) on the natural killer (NK) cytotoxic activity of peripheral blood mononuclear cells (PBMC) from patients with superficial and infiltrative transitional-cell carcinoma of the bladder (TCC) against both NK-sensitive and NK-resistant target cells. The normal NK activity found in PBMC from these patients can be significantly enhanced by short-term incubation (18 h) with INF beta (P < 0.05). The depressed NK cytotoxic activity found in PBMC from patients with infiltrative TCC can also be significantly enhanced, but not normalized, by short-term incubation with INF beta (P < 0.05). In kinetic studies we found that the maximal levels of the INF beta-promoted cytotoxic activity against NK-sensitive and against NK-resistant target cells in PBMC from TCC patients were reached after 18 h of culture. Short-term-INF beta-incubated PBMC from patients with TCC of the bladder also showed marked cytotoxic activity against NK-resistant target cells. The effector cells of the INF beta-induced cytotoxic activity in PBMC from patients with TCC were CD16+ CD3- NK cells. This cytotoxic inducer effect of INF beta synergized with that of interleukin-2. In conclusion, INF beta can enhance the NK activity of PMBC from patients with TCC of the bladder.
我们研究了β干扰素（INFβ）对膀胱浅表性和浸润性移行细胞癌（TCC）患者外周血单个核细胞（PBMC）的自然杀伤（NK）细胞毒性活性的影响，该活性针对NK敏感和NK抵抗靶细胞。这些患者PBMC中发现的正常NK活性可通过与INFβ短期孵育（18小时）而显著增强（P < 0.05）。浸润性TCC患者PBMC中降低的NK细胞毒性活性也可通过与INFβ短期孵育而显著增强，但不能恢复正常（P < 0.05）。在动力学研究中，我们发现培养18小时后，TCC患者PBMC中INFβ促进的针对NK敏感和NK抵抗靶细胞的细胞毒性活性达到最高水平。膀胱TCC患者短期经INFβ孵育的PBMC对NK抵抗靶细胞也显示出显著的细胞毒性活性。TCC患者PBMC中INFβ诱导的细胞毒性活性的效应细胞是CD16 + CD3 - NK细胞。INFβ的这种细胞毒性诱导作用与白细胞介素-2的作用协同。总之，INFβ可增强膀胱TCC患者PMBC的NK活性。
