Gupta S, Aggarwal S, Kim C, Gollapudi S
Division of Basic and Clinical Immunology, University of California, Irvine 92717.
Int J Immunopharmacol. 1994 Mar;16(3):197-204. doi: 10.1016/0192-0561(94)90013-2.
Human immunodeficiency virus 1 (HIV-1) and its purified proteins activate target cell functions. Because protein kinase C (PKC) plays a crucial role in signal transduction and there is a molecular heterogeneity of PKC, we compared the effect of recombinant HIV-1 gp120 and phorbol ester (PMA) on PKC isozymes in monocytic U937 cells, with isozyme-specific antibodies using flow cytometry. All PKC isozymes except PKC-gamma were present in U937 cells. Both PMA and HIV-1 gp120 increased levels of calcium-dependent and -independent PKC isozymes. The most striking change was observed in PKC-zeta isozymes levels. This study for the first time demonstrates that HIV-1 gp120 affects calcium-independent PKC isozymes in U937 cells.
人类免疫缺陷病毒1型（HIV-1）及其纯化蛋白可激活靶细胞功能。由于蛋白激酶C（PKC）在信号转导中起关键作用，且PKC存在分子异质性，我们使用同工酶特异性抗体，通过流式细胞术比较了重组HIV-1 gp120和佛波酯（PMA）对单核细胞U937细胞中PKC同工酶的影响。除PKC-γ外，所有PKC同工酶均存在于U937细胞中。PMA和HIV-1 gp120均可增加钙依赖性和非钙依赖性PKC同工酶的水平。PKC-ζ同工酶水平的变化最为显著。本研究首次证明HIV-1 gp120可影响U937细胞中钙非依赖性PKC同工酶。
