De Krey G K, Hollingshead N C, Kerkvliet N I, Smith B B
College of Veterinary Medicine, Oregon State University, Corvallis 97331.
Int J Immunopharmacol. 1994 Mar;16(3):251-7. doi: 10.1016/0192-0561(94)90020-5.
Halogenated aromatic hydrocarbons (HAH) are ubiquitous environmental contaminants. Studies in rats have shown that HAH treatment can lead to dysregulation of circulating hormone levels, including prolactin. Reduction of prolactin levels in both rats and mice is inhibitory to immune function. Previous studies have reported suppression of alloantigen-specific cytotoxic T-lymphocyte (CTL) activity in mice treated with 3,3', 4,4', 5,5'-hexachlorobiphenyl (HxCB). Here we report that treatment of mice with HxCB (10 mg/kg body weight) leads to a significant reduction of serum prolactin levels (by 89% to 3.7 ng/ml) on day 10 post alloantigen injection (P815 mastocytoma), the day of peak alloantigen-specific CTL activity. Prolactin levels were not altered on day 3 post alloantigen injection. Treatment with bromocriptine (5 mg/kg/day) reduced serum prolactin levels slightly on day 3 and significantly (94% to 2.1 ng/ml) on day 10 post alloantigen injection. Splenic CTL activity was not altered by treatment with bromocriptine. The data presented here suggest that reduction of prolactin levels alone, to the extent observed in HxCB-treated mice, is not causative for CTL suppression.
卤代芳烃（HAH）是普遍存在的环境污染物。对大鼠的研究表明，HAH处理可导致包括催乳素在内的循环激素水平失调。降低大鼠和小鼠体内的催乳素水平会抑制免疫功能。此前的研究报道，用3,3',4,4',5,5'-六氯联苯（HxCB）处理的小鼠，其同种异体抗原特异性细胞毒性T淋巴细胞（CTL）活性受到抑制。在此我们报告，在注射同种异体抗原（P815肥大细胞瘤）后第10天，即同种异体抗原特异性CTL活性达到峰值的那天，用HxCB（10毫克/千克体重）处理小鼠会导致血清催乳素水平显著降低（降低89%，至3.7纳克/毫升）。在注射同种异体抗原后第3天，催乳素水平未发生改变。用溴隐亭（5毫克/千克/天）处理，在注射同种异体抗原后第3天血清催乳素水平略有降低，在第10天则显著降低（降低94%，至2.1纳克/毫升）。溴隐亭处理未改变脾脏CTL活性。此处呈现的数据表明，仅将催乳素水平降低至HxCB处理小鼠中所观察到的程度，并非CTL抑制的原因。
