Kliffen M, Mooy C M, Luider T M, de Jong P T
Institute of Ophthalmology, Erasmus University, Rotterdam, The Netherlands.
Invest Ophthalmol Vis Sci. 1994 Jun;35(7):2901-5.
To analyze carbohydrate structures in basal laminar deposit (BLD), an extracellular material that accumulates between the retinal pigment epithelium (RPE) and Bruch's membrane. BLD has been shown to correlate positively with visual loss in age-related macular degeneration.
Thirteen postmortem human maculae with BLD were histochemically examined by light microscopy using the monoclonal antibody HNK-1 and seven lectins; canavalia ensiformis (ConA), soybean agglutinin (SBA), wheat germ agglutinin (WGA), dolichos bifloris (DBA), ulex europaeus (UEA-I), ricinius communis agglutinin I (RCA-I), and peanut agglutinin (PNA). Three maculae were stained with polyclonal antibodies against laminin and collagen type IV.
BLD was exclusively stained by DBA and SBA, whereas Con A, WGA, UEA-I, RCA-I, and HNK-1 stained various other structures in the human macula as well. The main part of the BLD adjacent to Bruch's membrane stained with these lectins and the monoclonal antibody HNK-1, whereas only a small part of the BLD adjoining the RPE stained with antibodies against laminin and collagen type IV. Drusen stained neither with any lectin nor with any antibody.
DBA and SBA, which bind specifically to an alpha-D-GalNAc moiety, are specific markers for the light-microscopic detection of BLD in human macular tissue. Furthermore, the authors conclude that BLD contains several carbohydrate structures other than the carbohydrate moieties on laminin and collagen type IV. If drusen contain carbohydrate structures, these must be different from those in BLD.
分析基底膜沉积物（BLD）中的碳水化合物结构。BLD是一种在视网膜色素上皮（RPE）和布鲁赫膜之间积累的细胞外物质。研究表明，BLD与年龄相关性黄斑变性中的视力丧失呈正相关。
对13个含有BLD的人死后黄斑进行组织化学检查，通过光学显微镜使用单克隆抗体HNK-1和七种凝集素；刀豆球蛋白A（ConA）、大豆凝集素（SBA）、麦胚凝集素（WGA）、双花扁豆凝集素（DBA）、欧洲荆豆凝集素（UEA-I）、蓖麻凝集素I（RCA-I）和花生凝集素（PNA）。用抗层粘连蛋白和IV型胶原的多克隆抗体对三个黄斑进行染色。
BLD仅被DBA和SBA染色，而ConA、WGA、UEA-I、RCA-I和HNK-1也对人黄斑中的各种其他结构进行染色。与布鲁赫膜相邻的BLD主要部分被这些凝集素和单克隆抗体HNK-1染色，而仅与RPE相邻的BLD一小部分被抗层粘连蛋白和IV型胶原的抗体染色。玻璃膜疣既不被任何凝集素也不被任何抗体染色。
特异性结合α-D-氨基半乳糖部分的DBA和SBA是人黄斑组织中BLD光学显微镜检测的特异性标志物。此外，作者得出结论，BLD除了含有层粘连蛋白和IV型胶原上的碳水化合物部分外，还含有几种碳水化合物结构。如果玻璃膜疣含有碳水化合物结构，这些结构必定与BLD中的不同。
