嗜酸性粒细胞颗粒主要碱性蛋白对角膜上皮伤口愈合的抑制作用。
Eosinophil granule major basic protein inhibition of corneal epithelial wound healing.
作者信息
Trocmé S D, Gleich G J, Kephart G M, Zieske J D
机构信息
Department of Ophthalmology, School of Medicine, University of Texas Medical Branch at Galveston 77555-0787.
出版信息
Invest Ophthalmol Vis Sci. 1994 Jun;35(7):3051-6.
PURPOSE
Human eosinophil major basic protein (MBP) was studied in an established organ culture model for rat corneal epithelial wound healing to elucidate further the role of the protein in vernal keratopathy.
METHODS
Epithelial migration rates were tested for five MBP concentrations (10, 25, 50, 100, and 200 micrograms/ml MBP).
RESULTS
Significantly less epithelial migration than control (P < 0.05) was observed in all tested groups. Histologic examination revealed abnormally heaped-up leading epithelial edges in all test groups compared to the normal tapered edges in all controls. Immunofluorescence disclosed MBP deposition on deepithelialized cornea.
CONCLUSIONS
These results suggest that MBP may contribute to vernal corneal ulcerations by inhibiting corneal epithelial migration.
目的
在已建立的大鼠角膜上皮伤口愈合器官培养模型中研究人嗜酸性粒细胞主要碱性蛋白（MBP），以进一步阐明该蛋白在春季角结膜炎中的作用。
方法
测试了五种MBP浓度（10、25、50、100和200微克/毫升MBP）下的上皮迁移率。
结果
在所有测试组中均观察到上皮迁移明显少于对照组（P < 0.05）。组织学检查显示，与所有对照组中正常的逐渐变细边缘相比，所有测试组中上皮前缘异常堆积。免疫荧光显示MBP沉积在去上皮化的角膜上。
结论
这些结果表明，MBP可能通过抑制角膜上皮迁移导致春季角膜溃疡。
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