Just W, De Almeida C C, Goldshmidt B, Vogel W
Abteilung Klinische Genetik, Universität Ulm, Germany.
Hereditas. 1994;120(1):71-5. doi: 10.1111/j.1601-5223.1994.00071.x.
The Polled mutation of the goat is inherited as an autosomal dominant trait. Homozygotes with a 60,XX karyotype develop into intersexes. We looked for the presence of Y-chromosomal sequences (Zfy and Sry) in a male pseudohermaphrodite polled goat by Southern blotting and PCR analysis. Molecular analysis revealed that this XX goat had no Sry sequence. It showed identical hybridization patterns, using the human SRY and ZFY probes at low stringency, with the normal XX female goat, whereas an XY male revealed prominent Sry and Zfy signals. Identical results were obtained from PCR analysis with the caprine Sry primers. The possible role of an autosomal recessive gene in the induction of testes and maleness is discussed.
山羊的无角突变作为常染色体显性性状遗传。具有60,XX核型的纯合子发育为雌雄间性。我们通过Southern印迹法和PCR分析，在一只雄性假两性畸形无角山羊中寻找Y染色体序列（Zfy和Sry）的存在。分子分析表明，这只XX山羊没有Sry序列。在低严谨度条件下，使用人类SRY和ZFY探针时，它与正常XX雌性山羊显示出相同的杂交模式，而XY雄性则显示出明显的Sry和Zfy信号。用山羊Sry引物进行PCR分析也得到了相同的结果。本文讨论了常染色体隐性基因在睾丸诱导和雄性化过程中的可能作用。
