Miyazaki T, Morimoto T, Fukuyama K, Matsubara H
Cereal Food R&D Section, Nisshin Flour Milling Co., Ltd., Saitama.
J Biochem. 1994 Feb;115(2):179-81. doi: 10.1093/oxfordjournals.jbchem.a124314.
The alpha-amylase inhibitor coded 0.19 (0.19AI) from wheat kernel is a dimeric protein which inactivates exogenous alpha-amylases. Crystals of 0.19AI were grown at room temperature and pH 7.0 from a protein solution with a low salt concentration. They were trigonal, belonged to space group P3(1) or P3(2), and had unit cell dimensions of a = b = 79.3 A and c = 60.8 A. The crystals diffract X-rays to at least 2.0 A resolution and are stable to X-ray beams. The asymmetric unit appears to contain two 0.19AI dimers. A potential heavy-atom derivative was prepared by soaking the crystal in a HgCl2 solution.
