Müthing J, Maurer U, Sostarić K, Neumann U, Brandt H, Duvar S, Peter-Katalinić J, Weber-Schürholz S
Institute for Cell Culture Technology, University of Bielefeld, Germany.
J Biochem. 1994 Feb;115(2):248-56. doi: 10.1093/oxfordjournals.jbchem.a124326.
The expression of neutral glycosphingolipids and gangliosides was investigated in mouse and rabbit skeletal muscle by means of biochemical and immunochemical techniques. Neutral glycosphingolipids from muscle of the inbred rabbit strain used in this study showed a simple TLC pattern, comprising mainly monohexosylceramide. In addition to this compound, lactosylceramide, lacto-N-neotetraosylceramide, globoside and Forssman GSL were detected in mouse muscle. The major ganglioside in both species was GM3; GM3 (Neu5Ac) and GM3(Neu5Gc) were found in a 3:1 ratio in mouse muscle, whereas the absence of GM3(Neu5Gc) is characteristic of rabbit muscle. As a general structural feature of all muscle GM3 gangliosides investigated, a C18 fatty acid and C18 sphingosine were the major components besides minor C22 and C24:1 fatty acids of the respective ceramide portions, as revealed by positive and negative ion FAB-MS. alpha 2-3 sialylated lacto-N-neotetraosyl-ceramide (sialylparagloboside) was expressed in both species, whereas the alpha 2-6 sialylated isomeric compound was found only in mouse muscle. Minute quantities of ganglio-series GM1, GD1a, GD1b, and GT1b were detected in muscles from both species. Glycosphingolipid expression could be confirmed immunohistochemically by examining transverse and longitudinal cryosections of skeletal muscle samples. The results provide the basis for the investigation of muscle specific glycosphingolipids that might modulate membrane protein functions in muscle.
通过生化和免疫化学技术研究了小鼠和兔骨骼肌中中性糖鞘脂和神经节苷脂的表达。本研究中使用的近交兔品系肌肉中的中性糖鞘脂呈现出简单的薄层层析图谱，主要由单己糖神经酰胺组成。除了这种化合物外，在小鼠肌肉中还检测到了乳糖基神经酰胺、乳糖-N-新四糖神经酰胺、红细胞糖苷脂和福斯曼糖鞘脂。两种物种中的主要神经节苷脂都是GM3；在小鼠肌肉中发现GM3（Neu5Ac）和GM3（Neu5Gc）的比例为3:1，而兔肌肉的特征是不存在GM3（Neu5Gc）。如正负离子快原子轰击质谱所示，作为所有研究的肌肉GM3神经节苷脂的一般结构特征，除了相应神经酰胺部分的少量C22和C24:1脂肪酸外，C18脂肪酸和C18鞘氨醇是主要成分。α2-3唾液酸化的乳糖-N-新四糖神经酰胺（唾液酸化副红细胞糖苷脂）在两种物种中均有表达，而α2-6唾液酸化的异构体化合物仅在小鼠肌肉中发现。在两种物种的肌肉中都检测到了微量的神经节系列GM1、GD1a、GD1b和GT1b。通过检查骨骼肌样品的横向和纵向冰冻切片，免疫组织化学可以证实糖鞘脂的表达。这些结果为研究可能调节肌肉膜蛋白功能的肌肉特异性糖鞘脂提供了基础。
