Karch F E, Morgan J P, Kubasik N P, Sine H E
J Clin Endocrinol Metab. 1976 Jul;43(1):26-31. doi: 10.1210/jcem-43-1-26.
Halofenate has been shown to decrease thyroxine (T4) binding to thyroxine-binding globulin (TBG) in vitro. Several indirect serum thyroid hormone assays are dependent on thyroid hormone binding and the results might be altered by halofenate in the serum. Halofenate free acid (50-500 mug/ml) was added to serum samples in vitro, and the samples were assayed for total serum T4 by competitive protein-binding assay (CPB) and radioimmunoassay (RIA), the per cent of dialyzable T4 (%FT4), total serum triiodothyronine (T3) by RIA, and resin T3 uptake (RT3U). Halofenate increased the measured T4 (CPB), %FT4, T3 (RIA), AND RT3U, but did not alter the T4 (RIA) determination. Thus, halofenate appears to diminish T4 binding to TBG in vitro, and artifactually alters the serum determinations of T4 (CPB), %FT4, T3 (RIA), AND RT3U. Calculated values derived from these measurements for the free thyroxine index and "free" T4 will also be affected halofenate. Only the T4 (RIA) determination was unchanged by the presence of halofenate in vitro.
已证实在体外，卤芬酯可减少甲状腺素（T4）与甲状腺素结合球蛋白（TBG）的结合。几种间接血清甲状腺激素检测依赖于甲状腺激素结合，血清中的卤芬酯可能会改变检测结果。将卤芬酯游离酸（50 - 500微克/毫升）体外加入血清样本中，通过竞争性蛋白结合分析法（CPB）和放射免疫分析法（RIA）检测样本中的血清总T4、可透析T4百分比（%FT4），通过RIA检测血清总三碘甲状腺原氨酸（T3）以及树脂T3摄取量（RT3U）。卤芬酯增加了所测的T4（CPB法）、%FT4、T3（RIA法）以及RT3U，但未改变T4（RIA法）的测定结果。因此，在体外卤芬酯似乎会减少T4与TBG的结合，并人为地改变血清中T4（CPB法）、%FT4、T3（RIA法）以及RT3U的测定结果。从这些测量值计算得出的游离甲状腺素指数和“游离”T4的值也会受到卤芬酯的影响。只有T4（RIA法）的测定在体外不受卤芬酯存在的影响。
