Fecho K, Maslonek K A, Coussons-Read M E, Dykstra L A, Lysle D T
Department of Psychology, University of North Carolina at Chapel Hill 27599.
J Immunol. 1994 Jun 15;152(12):5845-52.
The present study examined the role of macrophage-derived nitric oxide in the suppressive effect of in vivo morphine administration on Con A-stimulated proliferation of splenic lymphocytes in rats. The results showed that concentrations of nitrite are significantly greater in Con A-stimulated splenocyte cultures from morphine-treated rats than in cultures from saline-treated rats, and that the depletion of macrophages from splenocyte cultures abolishes the suppressive effect of morphine on Con A-stimulated proliferation. Moreover, the addition of NG-monomethyl-L-arginine (NMMA) to Con A-stimulated splenocyte cultures attenuates the suppressive effect of morphine on mitogenic responsiveness. The addition of excess L-arginine to splenocyte cultures containing NMMA reverses the effect of NMMA and restores morphine's suppressive effect on Con A-stimulated proliferation, but the addition of D-arginine to splenocyte cultures containing NMMA does not restore the suppressive effect of morphine. Taken together, these findings demonstrate that the suppressive effect of in vivo morphine administration on Con A-stimulated proliferation of splenic lymphocytes involves macrophage-derived nitric oxide.
本研究探讨了巨噬细胞衍生的一氧化氮在体内给予吗啡对大鼠脾脏淋巴细胞经刀豆蛋白A刺激的增殖的抑制作用中的作用。结果显示，经吗啡处理的大鼠的刀豆蛋白A刺激的脾细胞培养物中亚硝酸盐浓度显著高于经生理盐水处理的大鼠的培养物，并且从脾细胞培养物中去除巨噬细胞消除了吗啡对刀豆蛋白A刺激的增殖的抑制作用。此外，向刀豆蛋白A刺激的脾细胞培养物中添加NG-单甲基-L-精氨酸（NMMA）减弱了吗啡对有丝分裂反应性的抑制作用。向含有NMMA的脾细胞培养物中添加过量的L-精氨酸可逆转NMMA的作用并恢复吗啡对刀豆蛋白A刺激的增殖的抑制作用，但向含有NMMA的脾细胞培养物中添加D-精氨酸不能恢复吗啡的抑制作用。综上所述，这些发现表明体内给予吗啡对刀豆蛋白A刺激的脾脏淋巴细胞增殖的抑制作用涉及巨噬细胞衍生的一氧化氮。
